Morphologic transformation and chromosomal changes induced by chemical carcinogens in skin fibroblasts from patients with familial adenomatosis coli.
Morphologic transformation and chromosomal changes induced by chemical carcinogens in skin fibroblasts from patients with familial adenomatosis coli.
复制标题
化学致癌剂诱导家族性大肠杆菌腺瘤患者皮肤成纤维细胞的形态转变和染色体变化。
DOI：
10.1093/jnci/68.4.563
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
J. Utsunomiya
中科院分区：
文献类型：
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作者：
M. Miyaki;N. Akamatsu;T. Ono;A. Tonomura;J. Utsunomiya
来自家族性腺瘤病大肠杆菌（AC）患者和正常个体的皮肤成纤维细胞用致癌物4-硝基喹啉1-氧化物或N-甲基-N'-硝基-N-亚硝基胍处理一次，然后依次传代。在用致癌物处理后的第6-8代（第100-140天），在经致癌物处理的AC成纤维细胞的培养物中出现形态改变的细胞，但经致癌物处理的正常细胞和未经处理的AC和正常细胞即使在培养25个传代后也没有发生改变。含有形态改变细胞的培养物显示出转化细胞的特征，例如软琼脂糖中集落形成的频率高、生长能力增强和染色体异常。结果表明，AC 患者对化学致癌剂引起的形态转变和染色体变化的易感性增加。
Skin fibroblasts from patients with familial adenomatosis coli (AC) and normal individuals were treated once with the carcinogen 4-nitroquinoline 1-oxide or N-methyl-N'-nitro-N-nitrosoguanidine and then passaged sequentially. Morphologically altered cells appeared in the cultures of carcinogen-treated AC fibroblasts at passages 6-8 (days 100-140) after treatment with the carcinogens, but carcinogen-treated normal cells and untreated AC and normal cells did not become altered even after cultivation for 25 passages. The cultures containing morphologically altered cells showed characteristics of transformed cells, such as a high frequency of colony formation in soft agarose, increased growth ability, and chromosomal abnormalities. The results suggest tha AC patients have increased susceptibility to morphologic transformation and chromosomal changes induced by chemical carcinogens.