Characterization of a minimal simian virus 40 late promoter: enhancer elements in the 72-base-pair repeat not required.
Characterization of a minimal simian virus 40 late promoter: enhancer elements in the 72-base-pair repeat not required.
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最小猿猴病毒 40 晚期启动子的表征：不需要 72 碱基对重复中的增强子元件。
DOI：
10.1128/jvi.63.3.1420-1427.1989
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发表时间：
1989
影响因子：
5.4
通讯作者：
Chervitz,SA
中科院分区：
文献类型：
--
作者：
Dynan,WS;Chervitz,SA
猴病毒40调控区的272个碱基对（bp）部分包含复制起点、Sp1结合区和72 bp直接重复序列的一部分，构成了一个最小的晚期启动子，能够在体内和体外指导晚期方向RNA合成。该区域内的14个连接子扫描突变体的特征在于。Sp1结合区的突变降低了体内和体外的晚期表达。相比之下，消除早期转录增强子的遗传定义元件或阻止转录因子AP-1，AP-2和AP-3在72 bp重复区域中结合的突变对后期方向表达的影响很小或没有影响。这些结果表明，至少在某些情况下，早期转录增强子序列是不需要猿病毒40晚期基因表达。
A 272-base-pair (bp) portion of the simian virus 40 regulatory region containing the replication origin, Sp1-binding region, and part of the 72-bp direct repeats makes up a minimal late promoter that is able to direct late-direction RNA synthesis in vivo and in vitro. Fourteen linker-scan mutants within this region were characterized. Mutations in the Sp1-binding region decreased late expression both in vivo and in vitro. By contrast, mutations that eliminate genetically defined elements of the early transcriptional enhancer or that prevent binding of the transcription factors AP-1, AP-2, and AP-3 in the 72-bp repeat region had little or no effect on late-direction expression. These results argue that, at least under certain circumstances, the early transcriptional enhancer sequences are not required for simian virus 40 late gene expression.