3D printing hybrid organometallic polymer-based biomaterials via laser two-photon polymerization
3D printing hybrid organometallic polymer-based biomaterials via laser two-photon polymerization
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DOI：
10.1002/pi.5909
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发表时间：
2019-09-10
影响因子：
3.2
通讯作者：
Baltriukiene, Daiva
中科院分区：
文献类型：
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作者：
Balciunas, Evaldas;Baldock, Sara J.;Baltriukiene, Daiva
具有微尺度结构的材料由于其广泛的技术和医学应用而获得越来越多的兴趣。通过激光双光子聚合（也称为多光子制造）对这些物体进行增材制造的方法，可以创造出具有不同和可调特性的新材料。在这里，我们调查的3D结构组成的有机金属聚合物纳入铝，钛，钒和锆的属性。使用各种技术，包括扫描电镜，能量色散X射线光谱，X射线光电子能谱分析和接触角测量，并在体外测试其生物相容性的有机金属聚合物为基础的材料进行了分析。采用MTT法和吖啶橙子/溴化乙锭染色法检测细胞存活率和死亡方式。聚合物纳入铝，钛和锆支持细胞粘附和增殖，并显示出低毒性在体外，而有机金属聚合物纳入V被证明是细胞毒性。电感耦合等离子体发射光谱表明，V从有机金属聚合物中的浸出是可能的原因。有机金属聚合物的制备是简单的，简单的2D和复杂的3D结构都可以容易地制造。新开发的有机金属聚合物纳入铝的分辨率测试表明，悬浮线的宽度可达200 nm。我们相信，在这项工作中描述的材料显示出有前途的性能，为生物医学应用（如生物传感器，药物输送装置，组织支架等）的亚微米功能的对象的发展。(c)2019年，任作家。Polymer International，由John Wiley & Sons Ltd代表化学工业协会出版。
Materials with microscale structures are gaining increasing interest due to their range of technical and medical applications. Additive manufacturing approaches to such objects via laser two-photon polymerization, also known as multiphoton fabrication, enable the creation of new materials with diverse and tunable properties. Here, we investigate the properties of 3D structures composed of organometallic polymers incorporating aluminium, titanium, vanadium and zirconium. The organometallic polymer-based materials were analysed using a variety of techniques including SEM, energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy analysis and contact angle measurements and their biocompatibility was tested in vitro. Cell viability and mode of death were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and acridine orange/ethidium bromide staining. Polymers incorporating Al, Ti and Zr supported cell adhesion and proliferation, and showed low toxicity in vitro, whereas the organometallic polymer incorporating V was shown to be cytotoxic. Inductively coupled plasma optical emission spectrometry suggested that leaching of the V from the organometallic polymer is the likely cause of this. The preparation of the organometallic polymers is straightforward and both simple 2D and complex 3D structures can be fabricated with ease. Resolution tests of the newly developed organometallic polymer incorporating Al show that suspended lines with widths down to 200 nm can be fabricated. We believe that the materials described in this work show promising properties for the development of objects with sub-micron features for biomedical applications (e.g. biosensors, drug delivery devices, tissue scaffolds etc.). (c) 2019 The Authors. Polymer International published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.