COMPLEMENTARY PATTERNS OF EVEN-SKIPPED AND FUSHI-TARAZU EXPRESSION INVOLVE THEIR DIFFERENTIAL REGULATION BY A COMMON SET OF SEGMENTATION GENES IN DROSOPHILA
COMPLEMENTARY PATTERNS OF EVEN-SKIPPED AND FUSHI-TARAZU EXPRESSION INVOLVE THEIR DIFFERENTIAL REGULATION BY A COMMON SET OF SEGMENTATION GENES IN DROSOPHILA
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DOI：
10.1101/gad.1.9.981
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发表时间：
1987-11-01
影响因子：
10.5
通讯作者：
LEVINE, M
中科院分区：
文献类型：
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作者：
FRASCH, M;LEVINE, M
我们研究了包含同源盒的两个片段基因，even-skip （eve）和fushi tarazu （ftz）的位置依赖性表达。由这两个基因编码的产物在发育中的胚胎的前后体轴上形成一系列的七条横条纹。双染色实验表明eve和ftz蛋白在互补的胚胎细胞中积累。在发育过程中，共需要8个具有合子活性的分割基因来建立和完善eve和ftz的表达模式。人们发现，影响其中一个基因的突变总是会在另一个基因中产生相应的改变。这些结果表明，eve和ftz启动子独立地“解释”相同的位置线索，从而给出不同的表达模式。我们提出，激活eve表达的分割基因产物组合抑制了ftz的表达，反之亦然。这一建议是在分割基因之间相互作用的背景下进行讨论的，其中间隙基因在囊胚期胚胎中建立了eve和ftz表达的不对称模式，并且与其他配对规则基因的相互作用有助于进一步完善eve和ftz表达在原肠形成和胚带延伸期间的互补性。
We have examined the position-dependent expression of two segmentation genes that contain a homeo box, even-skipped (eve) and fushi tarazu (ftz). Products encoded by both genes accumulate in a series of seven transverse stripes along the anterior-posterior body axis of developing embryos. Double-staining experiments show that eve and ftz proteins accumulate in complementary sets of embryonic cells. A total of eight zygotically active segmentation genes are required for the establishment and refinement of the eve and ftz expression patterns during development. Mutations that affect one of the genes were always found to produce a reciprocal alteration in the other. These results suggest that the eve and ftz promoters independently "interpret" the same positional cues to give differential patterns of expression. We propose that a combination of segmentation gene products that activates the expression of eve represses the expression of ftz, and vice versa. This proposal is discussed in the context of a hierarchy of interactions among segmentation genes, whereby the gap genes establish asymmetric patterns of eve and ftz expression in blastoderm-stage embryos, and interactions with other pair-rule genes serve to refine further the complementarity of eve and ftz expression during gastrulation and germ band elongation.