Evaluation of photodynamic treatment using aluminum phthalocyanine tetrasulfonate chloride as a photosensitizer: new approach
Evaluation of photodynamic treatment using aluminum phthalocyanine tetrasulfonate chloride as a photosensitizer: new approach
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DOI：
10.1039/c2pp05411f
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Scalfi-Happ, Claudia
中科院分区：
文献类型：
--
作者：
Amin, Rehab M.;Hauser, Carmen;Scalfi-Happ, Claudia
光动力疗法(PDT)已经成为几项临床研究的主题。到目前为止的证据表明，细胞直接死亡可能涉及到细胞凋亡。T-24细胞(膀胱癌细胞，ATCC-Nr.HTB-4)在670 nm波长下，用四磺酸铝(AlS4Pc-Cl)和红色激光进行光动力转化。在共聚焦显微镜下观察PDT后的形态变化。拉曼显微光谱学被认为是检测细胞色素c作为细胞凋亡标志物的新方法之一。结果显示，3J cm(-2)照射后，经PDT处理的T-24细胞出现明显的细胞凋亡。经AlS4Pc-Cl孵育的细胞拉曼光谱检测不到细胞色素c，而AlS4Pc-Cl似乎干扰了细胞色素c的拉曼光谱。
Photodynamic therapy (PDT) has been the subject of several clinical studies. Evidence to date suggests that direct cell death may involve apoptosis. T-24 cells (bladder cancer cells, ATCC-Nr. HTB-4) were subjected to PDT with aluminum phthalocyanine tetrasulfonate chloride (AlS4Pc-Cl) and red laser light at 670 nm. Morphological changes after PDT were visualized under confocal microscopy. Raman microspectroscopy is considered as one of the newly established methods used for the detection of cytochrome c as an apoptotic marker. Results showed that PDT treated T-24 cells seem to undergo apoptosis after irradiation with 3 J cm(-2). Cytochrome c could not be detected from cells incubated with AlS4Pc-Cl using Raman spectroscopy whereas AlS4Pc-Cl seems to interfere with the Raman spectrum of cytochrome c.