The complete genomic sequence of the novel myovirus RP13 infecting Ralstonia solanacearum, the causative agent of bacterial wilt.
The complete genomic sequence of the novel myovirus RP13 infecting Ralstonia solanacearum, the causative agent of bacterial wilt.
复制标题
感染青枯菌（青枯病病原体）的新型肌病毒 RP13 的完整基因组序列。
DOI：
10.1007/s00705-020-04893-z
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yamada T.
中科院分区：
文献类型：
--
作者：
Kawasaki T;Endo H;Ogata H;Chatchawankanphanich O;Yamada T.
从泰国Pang Nga的番茄田中分离到一种新的裂解性噬菌体Ralstoniaphage RP 13。电镜观察表明，它具有新的三角形数（T = 21，右旋）的肌病毒的特征。RP13 DNA似乎被严重修饰。经RNA测序和RNA序列介导的DNA测序，RP 31基因组全长170，942 bp，平均G+C含量为39.2%。除了4个tRNA基因外，共有277个ORF被鉴定为结构、功能或假设基因。系统发育分析表明RP13与其他已知的哺乳动物没有密切关系。因此，我们得出结论，RP 13是一种新的噬菌体感染R。青枯菌是一种有效的青枯病生防菌。
A novel lytic bacteriophage,Ralstoniaphage RP13, was isolated from tomato fields in Pang Nga, Thailand. Electron microscopic observation showed it to have the features of a myovirus with a novel triangulation number (T = 21,dextro). The RP13 DNA appeared to be heavily modified. By applying RNA sequencing and RNA-sequence-mediated DNA sequencing, the whole genome of RP31 was determined to be 170,942 bp in length with a mean G+C content of 39.2%. A total of 277 ORFs were identified as structural, functional, or hypothetical genes in addition to four tRNA genes. Phylogenetic analysis suggested that RP13 is not closely related to any other known phages. Thus, we concluded that the RP13 is a novel phage infectingR. solanacearumstrains and will be a useful biocontrol agent against bacterial wilt disease.