TYPE-I CORTICOSTEROID RECEPTOR-LIKE IMMUNOREACTIVITY IN THE RAT CNS - DISTRIBUTION AND REGULATION BY CORTICOSTEROIDS
TYPE-I CORTICOSTEROID RECEPTOR-LIKE IMMUNOREACTIVITY IN THE RAT CNS - DISTRIBUTION AND REGULATION BY CORTICOSTEROIDS
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DOI：
10.1002/cne.903130312
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发表时间：
1991-11-15
影响因子：
2.5
通讯作者：
HARLAN, R
中科院分区：
文献类型：
--
作者：
AHIMA, R;KROZOWSKI, Z;HARLAN, R
以前的地图I型皮质类固醇受体结合在大鼠中枢神经系统（CNS）显示，边缘区，下丘脑，脑室周围器官的受体分布有限。最近的研究表明，该受体的表达更广泛，在边缘系统、运动系统和感觉系统中具有高水平的I型受体mRNA。我们用两种抗人I型皮质类固醇受体cDNA肽段的抗血清，对大鼠中枢神经系统（CNS）I型皮质类固醇受体样免疫反应性（Type I-ir）的细胞内定位进行了区域分布和皮质类固醇调节。最高密度的免疫反应神经元观察到边缘区，isocortex，和一些丘脑核。运动，感觉和内脏系统往往表现出中等密度的免疫反应神经元。在一些纤维系统中观察到I-ir型胶质细胞，例如，胼胝体、内侧丘系、大脑脚、三叉神经脊束和脊髓索。在大多数神经元和神经胶质细胞中，I型免疫反应呈弥漫性核和细胞质分布。长期肾上腺切除减少免疫反应在大多数神经元和胶质细胞。长期肾上腺切除后，I型免疫反应神经元主要定位于细胞质中。核免疫反应保留在苍白球，三叉神经运动核，脊髓的第8和第9层的一些神经元。急性治疗皮质酮或醛固酮恢复神经元和神经胶质I型免疫反应的密度低于在完整的大鼠。
Previous maps of Type I corticosteriod receptor binding in the rat central nervous system (CNS) revealed a restricted distribution of the receptor in limbic regions, hypothalamus, and circumventricular organs. More recent studies have shown a more widespread expression of the receptor, with high levels of Type I receptor mRNA in limbic, motor, and sensory systems. We have used two antisera against peptide sequences derived from the cDNA of the human Type I corticosteriod receptor to map the regional distribution and corticosteriod regulation of the intracellular location of Type I corticosteriod receptor-like immunoreactivity (Type I-ir) in the rat CNS.Neurons showing Type I-ir were observed at all levels of the CNS. Highest densities of immunoreactive neurons were observed in limbic regions, isocortex, and some thalamic nuclei. Motor, sensory, and visceral systems often showed moderate densities of immunoreactive neurons. Type I-ir glia were observed in some fiber systems, e.g., corpus callosum, medial lemniscus, cerebral peduncles, spinal trigeminal tract, and funiculi of the spinal cord. In the majority of neurons and in glia, Type I-ir showed a diffusely nuclear and cytoplasmic location. Long-term adrenalectomy reduced immunoreactivity in most neurons and glia. Neuronal Type I-ir was localized mainly in the cytoplasm after long-term adrenalectomy. Nuclear immunoreactivity was retained in some neurons in the globus pallidus, motor trigeminal nucleus, and laminae 8 and 9 of the spinal cord. Acute treatment with corticosterone or aldosterone restored neuronal and glial Type I-ir to densities below that seen in intact rats.