Analysis of microbiota associated with peri-implantitis using 16S rRNA gene clone library.
Analysis of microbiota associated with peri-implantitis using 16S rRNA gene clone library.
复制标题
使用16S rRNA基因克隆文库分析与种植植物炎相关的微生物群。
DOI：
10.3402/jom.v2i0.5104
复制
发表时间：
2010-05-24
影响因子：
4.5
通讯作者：
Izumi Y
中科院分区：
文献类型：
--
作者：
Koyanagi T;Sakamoto M;Takeuchi Y;Ohkuma M;Izumi Y
种植体周围炎（PI）是一种导致骨结合种植体周围软硬组织破坏的炎性疾病。龈下微生物群似乎是种植体周围病变的原因，尽管PI中已报告了微生物群的复杂性，但尚未确定导致PI的微生物群。本研究的目的是使用16S rRNA基因克隆文库分析来确定具有PI、临床健康种植体和牙周炎影响的牙齿的受试者中的微生物群，以澄清微生物差异。三名受试者参加了本研究。根据临床和放射学检查评估牙齿和种植体周围的情况，并选择患病的种植体、临床健康的种植体和牙周病牙。使用无菌纸尖从最深的袋中采集龈下菌斑样本。使用16S rRNA基因克隆文库技术分析细菌的患病率和身份。从测序的335个克隆中共鉴定出112个不同的物种。在112种植物中，有51种（46%）为未栽培的草本类型，其中22种为新的草本类型。在PI、牙周炎和牙周健康种植体部位鉴定的细菌种类数分别为77、57和12。PI中的微生物群主要包括革兰氏阴性菌，与健康种植体和牙周炎相比，其组成更加多样化。仅在PI位点检测到绿黄菌门、细肠菌门和增效菌门，以及微小微单胞菌、口消化链霉菌、解乳酸假分支杆菌和摩尔氏梭杆菌。在种植体周围病变中，牙周致病菌水平较低，如牙龈卟啉单胞菌和伴放线菌聚集菌。与牙周炎和牙周健康牙齿相比，PI中的生物膜显示出更复杂的微生物群，其主要由革兰氏阴性厌氧菌组成。常见的牙周致病菌显示出较低的患病率，并确定了几种细菌作为候选病原体在PI。
Peri-implantitis (PI) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. The subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in PI, the microbiota responsible for PI has not been identified. The purpose of this study was to identify the microbiota in subjects who have PI, clinically healthy implants, and periodontitis-affected teeth using 16S rRNA gene clone library analysis to clarify the microbial differences. Three subjects participated in this study. The conditions around the teeth and implants were evaluated based on clinical and radiographic examinations and diseased implants, clinically healthy implants, and periodontally diseased teeth were selected. Subgingival plaque samples were taken from the deepest pockets using sterile paper points. Prevalence and identity of bacteria was analyzed using a 16S rRNA gene clone library technique. A total of 112 different species were identified from 335 clones sequenced. Among the 112 species, 51 (46%) were uncultivated phylotypes, of which 22 were novel phylotypes. The numbers of bacterial species identified at the sites of PI, periodontitis, and periodontally healthy implants were 77, 57, and 12, respectively. Microbiota in PI mainly included Gram-negative species and the composition was more diverse when compared to that of the healthy implant and periodontitis. The phyla Chloroflexi, Tenericutes, and Synergistetes were only detected at PI sites, as were Parvimonas micra, Peptostreptococcus stomatis, Pseudoramibacter alactolyticus, and Solobacterium moorei. Low levels of periodontopathic bacteria, such as Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, were seen in peri-implant lesions. The biofilm in PI showed a more complex microbiota when compared to periodontitis and periodontally healthy teeth, and it was mainly composed of Gram-negative anaerobic bacteria. Common periodontopathic bacteria showed low prevalence, and several bacteria were identified as candidate pathogens in PI.