Expression of gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor mRNAs in various non-reproductive human tissues
Expression of gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor mRNAs in various non-reproductive human tissues
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DOI：
10.1016/0304-3835(95)90203-1
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发表时间：
1995-11-27
期刊：
影响因子：
9.7
通讯作者：
Jennes, L
中科院分区：
文献类型：
--
作者：
Kakar, SS;Jennes, L
最近，已经报道了从人乳腺肿瘤细胞系（MCF-7）和卵巢肿瘤克隆促性腺激素释放激素（GnRH）受体，以及其在各种其他人肿瘤、肿瘤细胞系和生殖器官中的表达（Kakar et al.，梅尔. h Cell.内分泌学、106（1994）145-149）。在本研究中，我们研究了GnRH和GnRH受体mRNA在正常人非生殖组织中的表达。使用逆转录聚合酶链反应（RT-PCR）技术和特定的寡核苷酸引物来自胎盘GnRH cDNA序列，PCR产物的预期大小，从人的肝脏，心脏，骨骼肌，肾脏，胎盘，垂体。PCR产物的真实性通过以内部寡核苷酸引物为探针的Southern印迹分析来证实。类似地，使用选自人垂体GnRH受体cDNA序列的GnRH受体的特异性寡核苷酸引物，从人肝脏、心脏、骨骼肌、肾脏、胎盘和垂体扩增预期大小的PCR产物，并且这些产物在Southern印迹上与人GnRH受体cDNA强烈杂交。克隆和核苷酸序列的PCR产物的GnRH和GnRH受体从心脏显示相同的序列相比，人胎盘GnRH和垂体GnRH受体的cDNA，分别。这些数据首次证明了正常人类非生殖组织中存在GnRH和GnRH受体mRNA，并表明GnRH及其受体除了调节垂体前叶促性腺激素的分泌外，还可能以自分泌或旁分泌方式在调节细胞功能中发挥重要作用。
Recently, cloning of the gonadotropin-releasing hormone (GnRH) receptor from the human breast tumor cell line (MCF-7) and from an ovarian tumor, and its expression in various other human tumors, tumor cell lines and reproductive organs have been reported (Kakar et al., Mel. Cell. Endocrinol., 106 (1994) 145-149). In the present studies, we investigated the expression of GnRH and GnRH receptor mRNAs in normal human non-reproductive tissues. Using reverse transcriptase polymerase chain reaction (RT-PCR) techniques and specific oligonucleotide primers derived from the placental GnRH cDNA sequence, PCR products of the expected size were obtained from human liver, heart, skeletal muscle, kidney, placenta, and pituitary. The authenticity of the PCR products was confirmed by Southern blot analysis with an internal oligonucleotide primer as probe. Similarly, using specific oligonucleotide primers for the GnRH receptor selected from the human pituitary GnRH receptor cDNA sequence, PCR products of the expected size were amplified from human liver, heart, skeletal muscle, kidney, placenta, and pituitary, and these strongly hybridized with the human GnRH receptor cDNA on Southern blot. Cloning and nucleotide sequencing of the PCR products for the GnRH and GnRH receptor from heart revealed identical sequences when compared to the human placental GnRH and pituitary GnRH receptor cDNAs, respectively. These data demonstrate for the first time the existence of GnRH and GnRH receptor mRNAs in normal human non-reproductive tissues and suggest that GnRH and its receptor may play an important role in the regulation of cellular functions in an autocrine or paracrine manner, in addition to regulating the secretion of gonadotropins from the anterior pituitary.