Analysis of genetic diversity in Agave tequilana var. Azul using RAPD markers.
Analysis of genetic diversity in Agave tequilana var. Azul using RAPD markers.
复制标题
DOI：
10.1023/a:1017553107303
复制
发表时间：
2001-01-01
期刊：
影响因子：
1.9
通讯作者：
de la Vega, O. Martinez
中科院分区：
文献类型：
--
作者：
Gil Vega, K.;Gonzalez Chavira, M.;de la Vega, O. Martinez
根据墨西哥联邦法律，A.龙舌兰酒变种。阿苏尔是唯一允许用于生产任何龙舌兰酒的龙舌兰品种。本研究的目的是分析田间种群的遗传变异水平。龙舌兰酒湾阿苏尔使用随机扩增多态性DNA（RAPD）标记。从四个不同的田地中的每一个收集十株植物，其中两个田地位于墨西哥的两个主要区域中的每一个用于栽培A.龙舌兰酒湾阿苏尔。这两个地区在地理上相隔约100公里。A.之间的遗传关系龙舌兰酒湾阿苏尔和A.龙舌兰酒'Chato'和'Siguin'也使用RAPD进行了研究。19个引物共扩增出130个标记，其中20个（15.4%）在A.龙舌兰酒湾Chato和A.龙舌兰酒湾西甘RAPD分析结果表明，A.龙舌兰酒湾Siguin与A.龙舌兰酒湾阿苏尔比A.龙舌兰酒湾查托在A.龙舌兰酒湾阿苏尔的124个RAPD扩增产物中只有1个多态性条带（0.8%），40个植株中有39个完全同基因。这是迄今为止在作物物种分析中检测到的最低水平的多态性之一，并且被认为是由于专门依赖营养繁殖来生产新的种植材料而多年来促进单一保守基因型的结果。这些结果的意义进行了讨论，在育种计划的重点是改善A。龙舌兰酒湾阿苏尔。
By federal law in Mexico, A. tequilana var. Azul is the only variety of agave permitted for the production of any tequila. Our objective was to assay levels of genetic variation in field populations of A. tequilana var. Azul using randomly amplified polymorphic DNA (RAPD) markers. Ten plants were collected from each of four different fields, with two fields being located in each of two principal regions of Mexico for the cultivation of A. tequilana var. Azul. The two regions are separated geographically by approximately 100 km. Genetic relationships between A. tequilana var. Azul and two other varieties of A. tequilana 'Chato' and 'Siguin', were also investigated using RAPDs. Among the three varieties, 19 decamer primers produced 130 markers, of which 20 (15.4%) were polymorphic between A. tequilana var. Chato and A. tequilana var. Siguin. The results of RAPD analysis suggest that A. tequilana var. Siguin is more closely related to A. tequilana var. Azul than is A. tequilana var. Chato. Among the 40 field selections of A. tequilana var. Azul, only 1 of 124 RAPD products (0.8%) was polymorphic and 39 of 40 plants were completely isogenic. This is one of the lowest levels of polymorphism detected to date for the analysis of a crop species, and is proposed to be the result of the promotion of a single conserved genotype over many years due to an exclusive reliance on vegetative propagation for the production of new planting materials. The significance of these results is discussed in relation to breeding programmes focused on the improvement of A. tequilana var. Azul.