Immunohistochemical distribution of delta opioid receptors in the rat central nervous system: Evidence for somatodendritic labeling and antigen-specific cellular compartmentalization
Immunohistochemical distribution of delta opioid receptors in the rat central nervous system: Evidence for somatodendritic labeling and antigen-specific cellular compartmentalization
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DOI：
10.1002/cne.1370
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发表时间：
2001-11-05
影响因子：
2.5
通讯作者：
Beaudet, A
中科院分区：
文献类型：
--
作者：
Cahill, CM;McClellan, KA;Beaudet, A
许多研究报道了哺乳动物中枢神经系统(CNS)中增量阿片受体(Delta OR)的分布。然而，由于免疫组织化学文献中的不一致，关于该受体的定位还没有普遍的共识。在本研究中，我们使用两种不同的抗体(针对大鼠Delta OR的C-末端或N-末端的序列)分析了免疫反应阳性的Delta OR在大鼠中枢神经系统中的细胞和亚细胞分布。通过使用Western blotting，这两种抗体在转导该受体的COS-7细胞中识别相似形式的Delta OR，但在大鼠脊髓的膜中识别不同形式的Delta OR。应用光镜免疫组织化学方法，两种抗体均可识别中枢神经系统中相同的神经细胞体；这些细胞体的分布与原位杂交检测到的Delta或mRNA表达细胞的分布一致。然而，C末端导向的抗体主要识别核周和近端的树突，而N末端导向的抗体也广泛标记树突和末梢乔木。此外，通过使用电子显微镜，发现这两种抗体不仅标记了躯体树突和轴突，而且还标记了质膜和细胞质，这表明不同的免疫形式的受体优先靶向不同的细胞和亚细胞区域。(C)2001年Wiley-Liss，Inc.
Many studies have reported on the distribution of delta opioid receptors (delta OR) in the mammalian central nervous system (CNS) by using a variety of techniques. However, no general consensus has emerged with regards to the localization of this receptor due to inconsistencies in the immunohistochemical literature. In the present study, we analyzed the cellular and subcellular distribution of immunoreactive delta OR in the rat CNS using two different antibodies (directed against a sequence in the C-terminus or N-terminus of the rat delta OR). By using Western blotting, these two antibodies recognized similar forms of the delta OR in COS-7 cells transfected with this receptor, but distinct forms in membranes from the rat spinal cord. By using light microscopic immunohistochemistry, both antibodies recognized identical populations of nerve cell bodies throughout the CNS; the distribution of these cell bodies conformed to that of delta OR mRNA-expressing cells detected by in situ hybridization. However, whereas the C-terminus-directed antibody recognized predominantly perikarya and proximal dendrites, the N-terminus-directed antibody also labeled extensively dendritic and terminal arbors. Furthermore, by using electron microscopy, the two antibodies were found not only to label differentially somatodendritic versus axonal compartments, but also plasma membrane versus cytoplasmic ones, suggesting that distinct immunological forms of the receptor are being targeted preferentially to different cellular and subcellular domains. (C) 2001 Wiley-Liss, Inc.