HUMAN COMPLEX-II(SUCCINATE-UBIQUINONE OXIDOREDUCTASE) - CDNA CLONING OF IRON SULFUR(IP) SUBUNIT OF LIVER-MITOCHONDRIA
HUMAN COMPLEX-II(SUCCINATE-UBIQUINONE OXIDOREDUCTASE) - CDNA CLONING OF IRON SULFUR(IP) SUBUNIT OF LIVER-MITOCHONDRIA
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DOI：
10.1016/0006-291x(90)91916-g
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发表时间：
1990-01-15
影响因子：
3.1
通讯作者：
KASAHARA, M
中科院分区：
文献类型：
--
作者：
KITA, K;OYA, H;KASAHARA, M
复合物II（琥珀酸-泛醌氧化还原酶）是真核细胞和原核生物中线粒体三羧酸循环和有氧呼吸链的重要酶复合物。在本研究中，人肝线粒体中铁硫-亚基的氨基酸序列是从通过免疫筛选人肝×人肝分离的cDNA推导的。gtll cDNA文库。分离的克隆含有786个核苷酸的开放阅读框，编码252个氨基酸的成熟蛋白，分子量为28，804。氨基酸序列分析表明，该肽与牛心脏的同源性为94.1%，与大肠杆菌sdh B产物的同源性为50.8%。在三个富含半胱氨酸的簇周围发现了引人注目的序列保守性，这些簇被认为是该酶的铁硫中心。这是首次报道线粒体复合体II的cDNA序列。
Complex II (succinate-ubiquinone oxidoreductase) is an important enzyme complex of both the tricarboxylic acid cycle and of the aerobic respiratory chains of mitochondria in eukaryotic cell and prokaryotic organisms. In this study, the amino acid sequence of iron sulfur-subunit in human liver mitochondria was deduced from cDNA which was isolated by immunoscreening a human liver .times. gtll cDNA library. An isolated clone contains an open reading frame of 786 nucleotides and encodes a mature potein of 252 amino acids with a molecular weight of 28,804. The amino acid sequence was highly homologous with that of bovine heart (94.1%) which has been determined from the purified peptide and that of Escherichia coli sdh B product (50.8%). Striking sequence conservation was found around the three cysteine-rich clusters which have been thought to comprise the iron-sulfur centers of the enzyme. This is the first report on the cDNA sequence of mitochondrial complex II.