Lactobacillus rhamnosus Reduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats
Lactobacillus rhamnosus Reduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats
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DOI：
10.1155/2020/6108575
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发表时间：
2020-01-13
影响因子：
4
通讯作者：
Jenie, Betty S. L.
中科院分区：
文献类型：
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作者：
Farida, Eko;Nuraida, Lilis;Jenie, Betty S. L.
据观察，一些乳酸菌（LAB）是潜在的益生菌，具有降低空腹血糖（FBG）等功能特性，可作为一种有前途的高血糖管理方法。本研究探讨了鼠李糖乳杆菌 BSL 和鼠李糖乳杆菌 R23 降低链脲佐菌素 (STZ) 诱导的糖尿病大鼠 FBG 的能力和机制。每天给大鼠口服鼠李糖乳杆菌 BSL 和鼠李糖乳杆菌 R23 1 mL 细胞悬液 (109 CFU/mL)，持续 30 天。每三天记录一次体重（BW），每六天记录一次FBG。注射STZ后1周和处死前测量口服葡萄糖耐量试验(OGTT)。在第 0、15 和 30 天收集粪便样本，通过 PCR 检测 16S rRNA 进行 LAB 群体和鉴定。口服鼠李糖乳杆菌 BSL 和鼠李糖乳杆菌 R23 通过下调葡萄糖 6 磷酸酶 (G6pc) 表达分别降低 0.57 倍和 0.60 倍，从而降低 FBG 并改善葡萄糖耐量 (P < 0.05)。正常大鼠和糖尿病大鼠的血脂、BUN、肌酐、SGOT和SGPT差异有显着性（P < 0.05），但糖尿病大鼠之间差异不显着（P > 0.05）。两种菌株都能有效增加粪便乳酸菌数量。从粪便样本中分离出的乳酸菌的分子鉴定表明它们能够存活并通过消化道。这些结果表明，这两种菌株都具有控制血糖水平的能力，并成为控制高血糖和糖尿病的有前途的药物。
Some lactic acid bacteria (LAB) are observed to be potential probiotics with functional properties such as lowering fasting blood glucose (FBG), as a promising hyperglycemia management. This study investigated the ability and mechanism of Lactobacillus rhamnosus BSL and Lactobacillus rhamnosus R23 on lowering FBG in diabetic rats induced by streptozotocin (STZ). The rats were orally administered with L. rhamnosus BSL and L. rhamnosus R23 by giving 1 mL cell suspension (109 CFU/mL) daily for 30 days. The body weight (BW) was recorded once in three days, and FBG was recorded once in six days. An oral glucose tolerance test (OGTT) was measured 1 week after injection with STZ and before sacrifice. Fecal samples were collected on days 0, 15, and 30 for LAB population and identification, performed by PCR detecting 16S rRNA. Oral administration of L. rhamnosus BSL and L. rhamnosus R23 decreased FBG and improved glucose tolerance via downregulation of glucose-6-phosphatase (G6pc) expression by 0.57- and 0.60-fold change, respectively (P < 0.05). The lipid profiles, BUN, creatinine, SGOT, and SGPT were significantly (P < 0.05) different between normal and diabetic rats, but they were not significantly (P > 0.05) different among diabetic rats. Both strains were effective in increasing fecal LAB population. Molecular identification of the isolated LAB from fecal sample indicated that they were able to survive and pass through the digestive tract. These results suggested that both strains have the ability to manage blood glucose level and become a promising agent to manage hyperglycemia and diabetes.