Prolyl Endopeptidase (PREP) is Associated With Male Reproductive Functions and Gamete Physiology in Mice
Prolyl Endopeptidase (PREP) is Associated With Male Reproductive Functions and Gamete Physiology in Mice
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DOI：
10.1002/jcp.25178
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发表时间：
2016-03-01
影响因子：
5.6
通讯作者：
Diano, Sabrina
中科院分区：
文献类型：
--
作者：
Dotolo, Raffaele;Kim, Jung Dae;Diano, Sabrina
Prolyl endopeptidase(PREP)是一种丝氨酸蛋白酶，参与了多种生物过程，如多肽激素和神经肽的成熟和降解、学习和记忆、细胞增殖和分化以及葡萄糖代谢等。少数报告还表明，PREP参与了与男性和女性生殖相关的过程。在本工作中，我们检测了Prep在雄性生殖细胞中的分布，并研究了其基因敲除(Prep(GT/GT))对成年小鼠睾丸和精子的影响。该蛋白在野生型(Wt)小鼠的伸长精子细胞和管腔精子以及支持细胞、间质细胞和小管周细胞中表达和定位。PREP也在附睾头和中段精子中表达，而其余尾部区域的信号较弱。此外，对wt和prep(gt/gt)小鼠的睾丸重量、曲细精管组织学和附睾精子参数进行了评估：野生型睾丸的平均管径和管腔直径较大；此外，wt和prep(gt/gt)生精管的管腔成分也不同，因为wt中生精管的比例要高得多。这些结果首次表明，prep的表达可能是正常生殖功能所必需的，并提示该酶可能在小鼠精子发生和精子生理中发挥作用。J.细胞。物理。2016年，231：551-557。(C)2015年威利期刊公司。
Prolyl endopeptidase (PREP) is a serine protease which has been implicated in many biological processes, such as the maturation and degradation of peptide hormones and neuropeptides, learning and memory, cell proliferation and differentiation, and glucose metabolism. A small number of reports have also suggested PREP participation in both male and female reproduction-associated processes. In the present work, we examined PREP distribution in male germ cells and studied the effects of its knockdown (Prep(gt/gt)) on testis and sperm in adult mice. The protein is expressed and localized in elongating spermatids and luminal spermatozoa of wild type (wt) mice, as well as Sertoli, Leydig, and peritubular cells. PREP is also expressed in the head and midpiece of epididymal spermatozoa, whereas the remaining tail region shows a weaker signal. Furthermore, testis weight, histology of seminiferous tubules, and epididymal sperm parameters were assessed in wt and Prep(gt/gt) mice: wild type testes have larger average tubule and lumen diameter; in addition, lumenal composition of seminiferous tubules is dissimilar between wt and Prep(gt/gt), as the percentage of spermiated tubules is much higher in wt. Finally, total sperm count, sperm motility, and normal morphology are also higher in wt than in Prep(gt/gt). These results show for the first time that the expression of PREP could be necessary for a correct reproductive function, and suggest that the enzyme may play a role in mouse spermatogenesis and sperm physiology. J. Cell. Physiol. 231: 551-557, 2016. (c) 2015 Wiley Periodicals, Inc.