Sequence of 3,687 nucleotides from the 3' end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins.
Sequence of 3,687 nucleotides from the 3' end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins.
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仙台病毒基因组 RNA 3 端的 3,687 个核苷酸序列以及病毒 NP、P 和 C 蛋白的预测氨基酸序列。
DOI：
10.1093/nar/11.21.7317
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发表时间：
1983
影响因子：
14.9
通讯作者：
K. Iwasaki
中科院分区：
文献类型：
--
作者：
T. Shioda;Y. Hidaka;T. Kanda;H. Shibuta;A. Nomoto;K. Iwasaki
仙台病毒基因组（Z株）3'端3，687个核苷酸的序列通过分子克隆技术测定，随后进行快速序列分析。在该区域观察到两个大的开放阅读框，一个由1，572个核苷酸组成，另一个由1，704个核苷酸组成，即从基因组的3'端开始的OP-1和OP-2。根据观察到的序列预测基因产物的氨基酸序列。通过对病毒蛋白P、HN、Fo、NP和M的氨基酸组成的测定，我们得出结论，NP和P分别是OP-1和OP-2的基因产物。在OP-2的3'端最近端区域发现了一个由612个核苷酸组成的额外开放阅读框架（OP-3）。OP-3的预测产物被认为是病毒非结构蛋白C。在基因组的3'末端的51个核苷酸的前导序列和蛋白质NP和P的每个基因的3'和5'末端的共有序列被鉴定。
The sequence of 3,687 nucleotides from the 3' end of the Sendai virus genome (Z strain) was determined by a molecular cloning technique followed by rapid sequence analysis. Two large open reading frames, one consisting of 1,572 nucleotides and the other of 1,704 nucleotides, were observed in the region, that is OP-1 and OP-2 from the 3' end of the genome. The amino acid sequences of the gene products were predicted from the observed sequence. Determination of amino acid compositions of viral proteins, P, HN, Fo, NP and M, led us to conclude that NP and P are the gene products of OP-1 and OP-2, respectively. An additional open reading frame consisting of 612 nucleotides (OP-3) was discovered in the 3' most proximal region of OP-2. The predicted product of OP-3 was considered to be viral non-structural protein C. The leader sequence of 51 nucleotides at the 3' terminal of the genome and consensus sequences at 3' and 5' ends of each gene for proteins NP and P were identified.