ANTIGENIC DIFFERENTIATION OF STRAINS OF TURKEY RHINOTRACHEITIS VIRUS USING MONOCLONAL-ANTIBODIES
ANTIGENIC DIFFERENTIATION OF STRAINS OF TURKEY RHINOTRACHEITIS VIRUS USING MONOCLONAL-ANTIBODIES
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DOI：
10.1080/03079459308418919
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发表时间：
1993-01-01
期刊：
影响因子：
2.8
通讯作者：
CAVANAGH, D
中科院分区：
文献类型：
--
作者：
COOK, JKA;JONES, BV;CAVANAGH, D
制备了抗火鸡鼻气管炎病毒（TRTV）UK分离株的单克隆抗体（mAb）。那些病毒中和的选择和使用，与多克隆抗血清提出的每一个分离的火鸡，在交叉中和试验对TRTV株分离在英国和其他地方。虽然多克隆抗血清显示它们之间存在一定的多样性，但检查的所有菌株都属于一种血清型。在英国分离的TRTV株可以通过一些mAb与其他地方分离的TRTV株明确区分。1978年在南非和1985年在英国制造的TRTV分离株比几个月内在英国和法国制造的分离株更密切相关。从火鸡和鸡中分离的TRTV株不能区分。一些单克隆抗体被发现是组特异性的，因为它们中和了所有检查的TRTV株。所有mAb均为IgG1或IgG2a同种型，并识别表面G糖蛋白。
Monoclonal antibodies (mAbs) were prepared against one UK isolate of turkey rhinotracheitis virus (TRTV). Those which were virus-neutralizing were selected and used, together with polyclonal antisera raised to each isolate in turkeys, in cross-neutralization tests against TRTV strains isolated in the UK and elsewhere. Whilst the polyclonal antisera showed that there was some diversity between them, all strains examined belonged to one serotype. The TRTV strains isolated in the UK could clearly be differentiated from those isolated elsewhere by some of the mAbs. Isolates of TRTV made in South Africa in 1978 and UK in 1985 were more closely related than were isolates made in UK and France within a few months. TRTV strains isolated from turkeys and chickens could not be differentiated. Some mAbs were found to be group-specific in that they neutralized all TRTV strains examined. All mAbs were of either the IgG1 or IgG2a isotype and recognized the surface G glycoprotein.