In vitro culture of Safflower L-cv. Bhima:: initiation, growth optimization and organogenesis
In vitro culture of Safflower L-cv. Bhima:: initiation, growth optimization and organogenesis
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DOI：
10.1023/a:1026493616991
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发表时间：
1999-01-01
影响因子：
3
通讯作者：
Shitole, MG
中科院分区：
文献类型：
--
作者：
Nikam, TD;Shitole, MG
红花愈伤组织诱导及离体植株再生体系的研究。通过研究苗龄、培养基因素、生长调节剂和切除方向对器官发生的影响，优化了根、下胚轴、子叶和叶片外植体对马的影响。在培养基中添加生长素和细胞分裂素的比例为bbb1的培养基可以提高愈伤组织的生长速度；而对于2,4- d的比例< 1.34 ~ 11.41 μ M，培养基中添加的生长调节剂（IAA、NAA、BA和Kinetin）对所有外植体的愈伤组织诱导和再生均有效。愈伤组织可维持32个月以上。BA （4.43 μ M）和酪蛋白水解物（10 mg l(-1)）对下胚轴（3-6）和子叶（5-7）外植体以及子叶衍生愈伤组织（4-8）的生芽率最高。从5 ~ 7日龄苗子叶最基部切下的外植体比从较老苗或较远的切下部位切下的外植体产生更多的芽。外植体的极性放置抑制了茎的再生。NAA + BA培养的愈伤组织的茎部再生潜力可保持7个月。在三种培养基中，MS优于SH-M和B(5)。芽的生根效率不高；42%的芽在含蔗糖(7-8%)+ IAA （2.8-5.7 μ M）的MS培养基上生根。在愈伤组织介导的子叶上的芽和在蔗糖、IAA、NAA和IBA的生根培养基上的芽均能诱导出头状花序。发育良好的植株移栽田间，成功率为34%。
Callus induction and in vitro plantlet regeneration systems for safflower (Carthamus tinctorius L.) cv. Bhima using root, hypocotyl, cotyledon and leaf explants were optimized by studying the influence on organogenesis of seedling age, media factors, growth regulators and excision orientation. Supplementation of the medium with an auxin: cytokinin ratio > 1 enhanced the growth rate of callus cultures; however, for 2,4-D the ratio was < 1.34-11.41 mu M concentrations of growth regulators (IAA, NAA, BA and Kinetin) in the medium were found effective for callus induction and regeneration in all explants. The calli could be maintained over 32 months. BA (4.43 mu M) combined with casein hydrolysate (10 mg l(-1)) yielded the highest rate of shoot production on hypocotyl (3-6) and cotyledon (5-7) explants and cotyledonary derived callus (4-8). More shoots were produced on explants cut from the most basal region of cotyledons from 5 to 7-day-old seedlings than from older seedlings or more distal cut sites. Apolar placement of explants, inhibited shoot regeneration. The shoot regeneration potential remained upto 7 months in calli developed on NAA + BA. Of three media tested, MS was superior to SH-M and B(5). Rooting of shoots was not efficient; 42% of the shoots were rooted on MS medium containing sucrose (7-8%) + IAA (2.8-5.7 mu M). Capitula induction was observed in both callus mediated shoots on cotyledons and shoots on rooting medium with sucrose, IAA, NAA and IBA. Well developed plantlets were transferred to the field with a 34% success rate.