Inhibitory effect of glucose and phosphate on the second cleavage division of hamster embryos: is it linked to metabolism?
Inhibitory effect of glucose and phosphate on the second cleavage division of hamster embryos: is it linked to metabolism?
复制标题
葡萄糖和磷酸盐对仓鼠胚胎第二次卵裂的抑制作用：与新陈代谢有关吗？
DOI：
10.1093/oxfordjournals.humrep.a019013
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Bavister,BD
中科院分区：
文献类型：
--
作者：
Barnett,DK;Bavister,BD
在葡萄糖和无机磷酸盐（Glu/Pi）存在的情况下，胚胎不能在体外完成第二次卵裂分裂。胚胎可以从暴露于Glu/Pi 8小时（12：30-20：30小时）中恢复，尽管卵裂延迟10小时，并且继续发育到囊胚阶段的情况很少（2%）。2-细胞胚胎对Glu/Pi的脆弱性在细胞周期中发生变化，在-21：00 h发生卵裂之前最大：从12：30-14：30 h暴露于Glu/Pi允许94%的桑椹胚/囊胚发育，而从18：30-20：30 h暴露于Glu/Pi使桑椹胚/囊胚发育下降至48%。这种抑制作用也受到暴露时间和培养基能量底物组成的调节。目前尚不清楚Glu/Pi如何干扰裂解，但可能是代谢解释，因为（i）代谢抑制剂L-甘油醛，碘乙酸和胱胺加剧了这种作用，（ii）用罗丹明123和共聚焦显微镜观察到的活性线粒体的组织被Glu/Pi干扰。
Hamster embryos cannot complete the second cleavage division in vitro in the presence of glucose and inorganic phosphate (Glu/Pi). Embryos can recover from an 8 h (12: 30–20: 30 h) exposure to Glu/Pi, although cleavage is retarded by 10 h and continued development to the blastocyst stage is rare (2%). The vulnerability of 2-cell embryos to Glu/Pi changes during the cell cycle, being greatest just prior to cleavage which occurs at −21: 00 h: exposure to Glu/Pi from 12: 30–14: 30 h allowed 94% development to morulae/blastocysts while exposure from 18: 30–20: 30 h decreased morula/blastocyst development to 48%. This inhibitory effect is also modulated by the length of exposure and energy substrate composition of the culture medium. It is unclear how Glu/Pi interferes with cleavage, but a metabolic explanation is likely because (i) metabolic inhibitors L-glyceraldehyde, iodoacetate and cystamine exacerbated the effect and (ii) the organization of active mitochondria observed with Rhodamine 123 and confocal microscopy is disturbed by Glu/Pi.