Neuroanatomical changes in the rat bladder after bladder outlet obstruction.
Neuroanatomical changes in the rat bladder after bladder outlet obstruction.
复制标题
膀胱出口梗阻后大鼠膀胱的神经解剖学变化。
DOI：
10.1046/j.1464-410x.1998.00873.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Cunha,G
中科院分区：
文献类型：
--
作者：
Sutherland,RS;Baskin,LS;Kogan,BA;Cunha,G
目的探讨膀胱出口部分梗阻大鼠模型中膀胱神经支配的组织学变化。材料与方法48只成年雌性大鼠通过20 G血管导管结扎近端尿道使其膀胱出口部分梗阻; 18只假手术大鼠作为对照。 在1周、2周和4周后处死动物，并使用计算机化形态测量法评估其膀胱。免疫组化染色神经元蛋白基因产物9.5（PGP，一般的神经元标记物）和乙酰胆碱酯酶，肾上腺素能纤维和一氧化氮synthes. Results膀胱壁的变化梗阻后，膀胱体积和重量增加了六至七倍的酶组织化学染色。在所有采样时间，平滑肌肥大同样明显。膀胱测压显示膀胱容量和排尿压力的功能改变;梗阻动物的膀胱容量显著增加，排尿压力更高（梗阻，80-100 cmH 2 O;正常，30-40 cmH 2 O）。 梗阻性膀胱的神经元变化在平滑肌束内和周围的胆碱能和肾上腺素能神经递质系统中最为显著，其中染色少于对照动物。PGP免疫反应性略有增加。l‐arginine‐nitric oxide pathway appeared unperturbed after obstruction.ConclusionsThese组织学研究结果表明，神经病理性变化在膀胱出口梗阻后，包括逼尿肌不稳定，主要是胆碱能和肾上腺素能途径的解剖扰动的结果。
ObjectiveTo investigate the histological changes in bladder innervation in response to partial bladder outlet obstruction in a rat model.Materials and methodsForty‐eight adult female rats had their bladder outlet partially obstructed by ligating the proximal urethra over a 20 G angiocatheter; 18 sham‐operated rats served as controls. Animals were killed after 1, 2 and 4 weeks, and their bladders evaluated using computerized morphometry. Immunohistochemical staining for neuronal protein gene‐product 9.5 (PGP, a general neuronal marker) and enzyme histochemical staining of acetylcholinesterase, adrenergic fibres and nitric oxide synthase were performed.ResultsBladder wall changes after obstruction consisted of a six‐ to sevenfold increase in bladder volume and weight. Smooth muscle hypertrophy was evident equally at all sample times. Cystometry showed functional alterations in bladder capacity and voided pressures; obstructed animals had markedly increased bladder capacities and higher voiding pressures (obstructed, 80–100 cmH2O; normal, 30–40 cmH2O). Neuronal changes in the obstructed bladder were most dramatic within the cholinergic and adrenergic neurotransmitter systems within and surrounding the smooth muscle bundles, where there was less staining than in control animals. PGP immunoreactivity increased slightly. The l‐arginine‐nitric oxide pathway appeared unperturbed after obstruction.ConclusionsThese histological findings suggest that neuropathic changes in the bladder after outlet obstruction, including detrusor instability, are mainly the result of anatomical perturbations in the cholinergic and adrenergic pathways.