Effects of lithium chloride and retinoic acid on the expression of genes from the Xenopus laevis Hox 2 complex.
Effects of lithium chloride and retinoic acid on the expression of genes from the Xenopus laevis Hox 2 complex.
复制标题
氯化锂和视黄酸对非洲爪蟾 Hox 2 复合体基因表达的影响。
DOI：
10.1002/aja.1001940104
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
DeRobertis,EM
中科院分区：
文献类型：
--
作者：
Leroy,P;DeRobertis,EM
我们表明，非洲爪蟾有一个Hox 2复杂的，这个复杂的是强烈保守的哺乳动物的，无论是在结构和规则的空间和时间顺序表达的基因在发展的早期阶段。氯化锂和视黄酸，两种试剂，已知改变轴向图案的机构时，适用于非洲爪蟾胚胎，分别产生，减少后，但夸大的前结构和胚胎与截断的前结构的胚胎。我们在这里报告的影响，这些试剂的表达Hox 2基因在非洲爪蟾胚胎。LiCl对Hox基因有显著影响，在原肠胚形成和早期神经胚形成期间抑制这些基因的表达。然而，后来这些基因的表达达到显着的水平，表明存在两个阶段的控制Hox基因的表达。视黄酸增加了Hox基因转录物的稳态水平，对Hox 2.7影响最大，Hox 2.7是所研究的最前面的基因。这表明在EC细胞中获得的结果（Simeone等人，1990年，1991年）反映了体内发生的情况。氯化锂和RA都不改变基因表达开始的顺序，表明这些试剂不会干扰用于建立Hox复合物基因顺序激活的分子机制。o 1992年Wiley-Liss，Inc.
We show that Xenopus laevis has a Hox 2 complex, and that this complex is strongly conserved with the mammalian one, both in structure and in the rules of spatial and temporal sequential expression of its genes during the early stages of development. Lithium chloride and retinoic acid, two reagents known to alter axial patterning of the body when applied to Xenopus embryos, produce, respectively, embryos with reduced posterior but exaggerated anterior structures and embryos with truncation of anterior structures. We report here on the effect of these reagents on the expression of Hox 2 genes in the Xenopus embryo. LiCl has a dramatic effect on Hox genes, suppressing the expression of these genes during gastrulation and early neurulation. However, later on expression of these genes reaches significant levels, suggesting the existence of two phases in the control of Hox gene expression. Retinoic acid increases the steady state level of transcripts from Hox genes with the greatest effect on Hox 2.7, the most anterior of the genes studied. This suggests that the results obtained in EC cells (Simeone et al., 1990, 1991) reflect what occurs in vivo. Neither LiCl nor RA change the sequential order of the onset of expression of the genes, showing that these reagents do not perturb the molecular mechanisms used to establish the sequential activation of the genes of the Hox complexes. o 1992 Wiley-Liss, Inc.