The effect of repeated ozone exposures on inflammatory markers in bronchoalveolar lavage fluid and mucosal biopsies.
The effect of repeated ozone exposures on inflammatory markers in bronchoalveolar lavage fluid and mucosal biopsies.
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DOI：
10.1164/ajrccm.161.6.9908102
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发表时间：
2000-06
影响因子：
24.7
通讯作者：
R. Jörres;O. Holz;W. Zachgo;P. Timm;S. Koschyk;Bernd Müller;F. Grimminger;W. Seeger;F. Kelly;C. Dunster;T. Frischer;Gerd Lubec;Marc Waschewski;A. Niendorf;H. Magnussen
中科院分区：
文献类型：
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作者：
R. Jörres;O. Holz;W. Zachgo;P. Timm;S. Koschyk;Bernd Müller;F. Grimminger;W. Seeger;F. Kelly;C. Dunster;T. Frischer;Gerd Lubec;Marc Waschewski;A. Niendorf;H. Magnussen
本研究的目的是调查与反复接触臭氧有关的细胞和生化事件。23名健康受试者接受单次暴露于200 ppb臭氧和过滤空气（FA），以及连续4天重复暴露于200 ppb臭氧，每次间歇运动4小时。在单次或最后一次重复暴露后20小时进行支气管肺泡灌洗和粘膜活检。与FA相比，单次臭氧暴露导致FEV降低(1)，中性粒细胞和淋巴细胞的百分比增加，BAL液（BALF）中总蛋白、IL-6、IL-8、还原性谷胱甘肽、尿酸盐和正酪氨酸的浓度增加，但活检细胞组成没有变化。反复暴露后，对肺功能的影响消失，BALF中细胞计数差异与FA后无显著差异。但总蛋白、白细胞介素6、白细胞介素8、还原性谷胱甘肽和正酪氨酸的浓度仍然升高。IL-10只有在反复暴露于臭氧后才能在半叶中检测到。此外，支气管炎、红斑、气道黏膜超易感性的宏观评分增加，支气管粘膜活检中中性粒细胞数量增加。我们的数据表明，尽管BALF中的一些炎症标记物减弱并且肺功能适应，但反复暴露于臭氧后气道炎症持续存在。
The aim of this study was to investigate the cellular and biochemical events associated with repeated exposures to ozone. Twenty-three healthy subjects underwent single exposures to 200 ppb ozone and to filtered air (FA), as well as repeated exposures to 200 ppb ozone on 4 consecutive days, each for 4 h of intermittent exercise. Bronchoalveolar lavage was performed and mucosal biopsies were taken 20 h after the single or the last of the repeated exposures. As compared with FA, the single exposure to ozone caused a decrease in FEV(1), an increase in the percentages of neutrophils and lymphocytes, the concentrations of total protein, IL-6, IL-8, reduced glutathione, urate, and ortho-tyrosine in BAL fluid (BALF), but no changes in the cellular composition of biopsy. After the repeated exposure, the effect on lung function was abolished and differential cell counts in BALF were not significantly different from those after FA. However, the concentrations of total protein, IL-6, IL-8, reduced glutathione, and ortho-tyrosine were still increased. IL-10 could only be detected in BALF after repeated ozone exposures. Furthermore, macroscopic scores for bronchitis, erythema, and hypervulnerability of airway mucosa were increased, as well as numbers of neutrophils in bronchial mucosal biopsies. Our data demonstrate that airway inflammation persists after repeated ozone exposure, despite attenuation of some inflammatory markers in BALF and adaptation of lung function.