Salivary gland hyaluronidase in various species of phlebotomine sand flies (Diptera: Psychodidae)
Salivary gland hyaluronidase in various species of phlebotomine sand flies (Diptera: Psychodidae)
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DOI：
10.1016/s0965-1748(02)00109-1
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发表时间：
2002-12-01
影响因子：
3.8
通讯作者：
Volf, P
中科院分区：
文献类型：
--
作者：
Cerná, P;Mikes, L;Volf, P
对6种沙蝇雌性唾液腺提取物的透明质酸酶活性进行了检测和部分鉴定。在木瓜白血鱼和长掌Lutzomyia longipalpis中，该酶在较宽的pH范围内具有活性；最适pH为5.0。除对透明质酸具有较高的裂解活性外，还能水解硫酸软骨素A和c。不同种类沙蝇的透明质酸酶在结构和对还原条件的敏感性上存在差异。在Phlebotomus亚属（P. papatasi和P. duboscqi）和Adlerius亚属（P. halepensis）中，酶的主要活性形式是单体，在非还原和还原条件下具有相同的表观分子量（P. papatasi和P. duboscqi约为65 kDa， P. halepensis约为1 10 kDa）。在P. sergenti中，该酶作为假定的同二聚体出现，但当分离为60 kDa亚基时，在还原条件下仍保持活性。在长鼻L. longipalpis和perniciosus中，仅在非还原条件下检测到活性。在雄性中也发现了较低的酶活性。沙蝇唾液腺透明质酸酶与哺乳动物精子细胞内切- n -乙酰-己糖氨酸酶及膜翅目昆虫相应的毒液酶具有共性。据推测，它们不仅可以促进血液获取，还可以调节宿主的免疫反应，促进病原体的传播。(C) 2002 Elsevier Science Ltd.版权所有。
Hyaluronidase activity was detected and partially characterized in salivary gland extracts of females of six sand fly species. In Phlebotomus papatasi and Lutzomyia longipalpis the enzyme was active over a broad pH range; the pH optimum was 5.0. Besides high cleaving activity towards hyaluronic acid, it hydrolyzed chondroitin sulfates A and C. Hyaluronidases of various sand fly species differed in structure and sensitivity to reducing conditions. In the subgenera Phlebotomus (P. papatasi and P. duboscqi) and Adlerius (P. halepensis) the predominant active form of the enzyme was monomeric with the same apparent molecular weight under nonreducing and reducing conditions (around 65 kDa for P. papatasi and P. duboscqi and I 10 kDa for P. halepensis). In P. sergenti the enzyme occurred as a putative homodimer but remained active under reducing conditions when separated into 60 kDa subunits. In L. longipalpis and P. perniciosus the activity was detectable under non-reducing conditions only. In P. duboscqi, low enzyme activity was found also in males. Salivary gland hyaluronidases of sand flies share characteristics with endo-N-acetyl-hexosaminidases of mammalian sperm cells and corresponding venom enzymes of Hymenoptera. Hypothetically, they facilitate blood meal acquisition but also may modulate immune reactions of the host and promote pathogen transmission. (C) 2002 Elsevier Science Ltd. All rights reserved.