Highly efficient detection of paclobutrazol in environmental water and soil samples by time-resolved fluoroimmunoassay
Highly efficient detection of paclobutrazol in environmental water and soil samples by time-resolved fluoroimmunoassay
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时间分辨荧光免疫分析法高效检测环境水和土壤样品中的多效唑
DOI：
10.1016/j.scitotenv.2016.06.089
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发表时间：
2016-11-01
影响因子：
9.8
通讯作者：
Du, Daolin
中科院分区：
文献类型：
--
作者：
Liu, Zhenjiang;Wei, Xi;Du, Daolin
建立了一种快速、灵敏的间接竞争时间分辨荧光免疫分析方法(TRFIA)，用于环境水样和土壤样品中多效唑的分析。合成了多效唑半抗原，并将其与牛血清白蛋白(BSA)偶联制备多克隆抗体。在最佳条件下，该方法的半数抑制浓度(IC50值)为1.09mgL-1，检出限(LOD值)为0.067 mgL-1。与已报道的ELISA法相比，TRFIA的LOD提高了30倍。该抗体与三氮唑类化合物的其他结构类似物几乎没有交叉反应，表明该抗体具有较高的特异性。加标样品的平均回收率为80.2%~104.7%，相对标准偏差为1.0%~9.5%。实际样品的TRFIA结果与高效液相色谱分析结果一致。结果表明，建立的TRFIA方法在环境样品中多效唑的检测中具有潜在的应用价值。(C)2016爱思唯尔B.V.保留所有权利。
A fast and ultrasensitive indirect competitive time-resolved fluoroimmunoassay (TRFIA) was developed for the analysis of paclobutrazol in environmental water and soil samples. Paclobutrazol hapten was synthesized and conjugated to bovine serum albumin (BSA) for producing polyclonal antibodies. Under optimal conditions, the 50% inhibitory concentration (IC50 value) and limit of detection (LOD, IC20 value) were 1.09 mu g L-1 and 0.067 mu g L-1, respectively. The LOD of TRFIA was improved 30-fold compared to the already reported ELISA. There was almost no cross-reactivity of the antibody with the other structural analogues of triazole compounds, indicating that the antibody had high specificity. The average recoveries from spiked samples were in the range from 80.2% to 104.7% with a relative standard deviation of 1.0-9.5%. The TRFIA results for the real samples were in good agreement with that obtained by high-performance liquid chromatography analyses. The results indicate that the established TRFIA has potential application for screening paclobutrazol in environmental samples. (C) 2016 Elsevier B.V. All rights reserved.