Engineering of Reversible NIR-II Redox-Responsive Fluorescent Probes for Imaging of Inflammation In Vivo
Engineering of Reversible NIR-II Redox-Responsive Fluorescent Probes for Imaging of Inflammation In Vivo
复制标题
用于体内炎症成像的可逆 NIR II 氧化还原响应荧光探针的工程
DOI：
10.1002/anie.202211409
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发表时间：
2022-10-17
影响因子：
16.6
通讯作者：
Yuan, Lin
中科院分区：
文献类型：
--
作者：
He, Long;He, Lin-Hui;Yuan, Lin
二次近红外荧光成像由于其自身荧光背景低、光子散射小等优点，在活体组织的高分辨率分析中具有巨大的应用潜力。然而，由于缺乏合适的探针，使用NIR-II荧光成像可逆监测氧化还原稳态仍然是一个挑战。本研究以三次甲基为骨架，构建了一系列稳定的多功能近红外-Ⅱ染料（NIR-IICy 3s）。值得注意的是，将1，4-二乙基-十氢喹喔啉基团引入NIR-II Cy 3不仅有效地增加了波长，而且还作为HClO的有效响应位点，其可以通过活性硫物种（RSS）恢复。在此基础上，NIR-II Cy 3s用于病理生理学环境中HClO/RSS介导的氧化还原过程的可逆监测。最后，NIR-II Cy 3 -988被成功地用于评估急性炎症模型中的氧化还原环境和药物治疗效果。
The second near-infrared (NIR-II) fluorescent imaging shows great potential for deep tissue analysis at high resolution in living body owing to low background autofluorescence and photon scattering. However, reversible monitoring of redox homeostasis using NIR-II fluorescent imaging remains a challenge due to the lack of appropriate probes. In this study, a series of stable and multifunctional NIR-II dyes (NIR-II Cy3s) were constructed based on trimethine skeleton. Significantly, introducing the 1,4-diethyl-decahydroquinoxaline group to the NIR-II Cy3s not only effectively increased the wavelength, but also served as an effective response site for HClO, which can be restored by reactive sulfur species (RSS). Based on this, NIR-II Cy3s were used for reversible monitoring of HClO/RSS-mediated redox processes in the pathophysiology environment. Finally, NIR-II Cy3-988 was successfully utilized for assessment of the redox environments and drug treatment effects in acute inflammation model.