Sustained release of adiponectin improves osteogenesis around hydroxyapatite implants by suppressing osteoclast activity in ovariectomized rabbits.
Sustained release of adiponectin improves osteogenesis around hydroxyapatite implants by suppressing osteoclast activity in ovariectomized rabbits.
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DOI：
10.1016/j.actbio.2011.10.029
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发表时间：
2012-02
影响因子：
9.7
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Luo E;Hu J;Bao C;Li Y;Tu Q;Murray D;Chen J
缺乏雌激素可导致骨量减少和骨质疏松症风险增加，这对生物材料植入有负面影响。脂联素（APN）是一种脂肪源性激素，通过抑制破骨细胞的形成和促进成骨细胞的形成来增加骨密度。本研究旨在观察Matrigel控释系统释放的APN对体外培养的兔成熟破骨细胞和破骨细胞前体RAW264.7细胞活性的直接影响，并通过促进卵巢切除（OVX）兔羟基磷灰石（HA）植入物周围成骨来确定其效果。将APN+Matrigel+HA、APN+HA、Matrigel+HA和HA植入OVX兔下颌骨缺损区。术后4周行组织学检查、微电脑断层扫描及生物力学测试。结果表明，Matrigel延长了APN释放的长度，以实现长期持久性。APN缓释剂在体内外均能抑制种植体的成骨活性，促进OVX兔种植体周围成骨，而短期APN处理则无此作用。APN的持续释放可能是一种有效的策略，以改善骨缺损的修复，通过使用HA材料在破骨细胞高度活化的骨质疏松条件下。
Lack of estrogen could lead to decreased bone mass and increased risk for osteoporosis, which has a negative influence on biomaterial implantation. Adiponectin (APN), an adipose-derived hormone, has been shown to increase bone density by inhibiting osteoclast formation and promoting the formation of osteoblasts. This study was designed to investigate the direct effects of APN released from the Matrigel controlled-release system on the activity of rabbit mature osteoclasts and osteoclast precursor RAW264.7 cells in vitro, and to determine its effects by improving osteogenesis around the hydroxyapatite (HA) implant in ovariectomized (OVX) rabbits. APN+Matrigel+HA, APN+HA, Matrigel+HA and HA were implanted into mandibular defects of OVX rabbits. At 4weeks after implantation, the mandibles were examined by histology, microcomputed tomography and biomechanical testing. The results demonstrated that Matrigel extended the length of APN released to achieve long-term persistence. The sustained release of APN suppressed the osteoclastic activity both in vitro and in vivo, and improved the peri-implant osteogenesis in OVX rabbits, while the short-term APN treatment did not. Sustained release of APN may be an effective strategy to improve the restoration of bone defects by the use of HA materials under osteoporotic conditions in which osteoclasts are highly activated.