Molecular cloning of a peroxisomal Ca2+-dependent member of the mitochondrial carrier superfamily.
Molecular cloning of a peroxisomal Ca2+-dependent member of the mitochondrial carrier superfamily.
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线粒体载体超家族过氧化物酶体 Ca2 依赖性成员的分子克隆。
DOI：
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发表时间：
1997
影响因子：
11.1
通讯作者：
H. Hauser
中科院分区：
文献类型：
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作者：
F. E. Weber;G. Minestrini;J. Dyer;M. Werder;D. Boffelli;S. Compassi;E. Wehrli;R. Thomas;G. Schulthess;H. Hauser
从兔小肠cDNA文库中分离到一个新的线粒体溶质载体超家族钙依赖性成员的cDNA。全长cDNA克隆为3，298 nt长，编码475个氨基酸的蛋白质，在分子的N-末端的一半具有四个延伸因子-手基序。在大肠杆菌中表达的25-kDa的N-末端多肽，它被证明，它结合Ca 2+，经历了可逆的和特定的构象变化的结果。多肽的构象对以高亲和力结合的Ca 2+敏感（Kd约0.37 μ M），Ca 2+诱导的变化的表观希尔系数约为2.0。推导的氨基酸序列的C-末端的一半的分子揭示78%的同源性，格雷夫斯病载体蛋白和67%的同源性，人类ADP/ATP转位酶，这一序列的同源性确定的蛋白质作为线粒体转运蛋白超家族的新成员。北方印迹分析显示存在约3，500个碱基的单个转录物，并且可以在肾脏中检测到转运蛋白的低表达，但在肝脏中没有。主要表达部位是结肠，在回肠近端的小肠中发现少量表达。免疫电子显微镜定位的过氧化物酶体的转运，虽然在线粒体中发现了一小部分。Ca 2+结合转运蛋白的N-末端的一半面向胞质溶胶。
A cDNA from a novel Ca2+-dependent member of the mitochondrial solute carrier superfamily was isolated from a rabbit small intestinal cDNA library. The full-length cDNA clone was 3,298 nt long and coded for a protein of 475 amino acids, with four elongation factor-hand motifs located in the N-terminal half of the molecule. The 25-kDa N-terminal polypeptide was expressed in Escherichia coli, and it was demonstrated that it bound Ca2+, undergoing a reversible and specific conformational change as a result. The conformation of the polypeptide was sensitive to Ca2+ which was bound with high affinity (Kd approximately 0.37 microM), the apparent Hill coefficient for Ca2+-induced changes being about 2.0. The deduced amino acid sequence of the C-terminal half of the molecule revealed 78% homology to Grave disease carrier protein and 67% homology to human ADP/ATP translocase; this sequence homology identified the protein as a new member of the mitochondrial transporter superfamily. Northern blot analysis revealed the presence of a single transcript of about 3,500 bases, and low expression of the transporter could be detected in the kidney but none in the liver. The main site of expression was the colon with smaller amounts found in the small intestine proximal to the ileum. Immunoelectron microscopy localized the transporter in the peroxisome, although a minor fraction was found in the mitochondria. The Ca2+ binding N-terminal half of the transporter faces the cytosol.
通过亚微摩尔钙浓度控制线粒体腺嘌呤核苷酸含量及其与激素效应的关系。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
HaynesJr,RC;Picking,RA;Zaks,WJ
通讯作者：
Zaks,WJ
DeHydroepiandrosterone 3 beta-sulfate 是过氧化物酶体增殖途径的内源性激活剂：在原代大鼠肝细胞培养物中诱导细胞色素 P-450 4A 和酰基辅酶 A 氧化酶 mRNA 以及 Ca(2 ) 通道阻滞剂的抑制作用。
DOI：
10.1042/bj3010753
发表时间：
1994
期刊：
The Biochemical journal
影响因子：
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作者：
Ram,PA;Waxman,DJ
通讯作者：
Waxman,DJ