IL-2 and IL-5 gene expression in response to alloantigen in liver allograft recipients and in vitro.
IL-2 and IL-5 gene expression in response to alloantigen in liver allograft recipients and in vitro.
复制标题
在同种异体肝移植受者和体外，IL-2 和 IL-5 基因表达对同种异体抗原的反应。
DOI：
10.1097/00007890-199305000-00042
复制
发表时间：
1993
期刊：
影响因子：
6.2
通讯作者：
Krams,SM
中科院分区：
文献类型：
--
作者：
Martinez,OM;Villanueva,JC;Lake,J;Roberts,JP;Ascher,NL;Krams,SM
在同种异体肝移植受者和体外系统中研究了同种异体抗原响应的 IL-2 和 IL-5 基因表达。通过聚合酶链反应和 Southern 印迹杂交分析了来自 22 名患者的 77 个连续同种异体肝活组织检查中的 IL-2 和 IL-5 mRNA。 74% 的同种异体移植物中存在 IL-5 信息，46% 的同种异体移植物中存在排斥反应，33% 的同种异体移植物中没有排斥反应。排斥同种异体移植物中IL-5转录物的频率与无排斥证据的移植物中IL-5转录物的频率显着不同(P=0.003)。在 29% 的排斥同种异体移植物中检测到 IL-2 信息，在 18% 的无排斥证据的同种异体移植物中检测到 IL-2 信息，在 43% 的同种异体移植物中检测到排斥反应已解决。 IL-2基因表达与同种异体移植物的组织病理学状态之间没有显着相关性。有趣的是，在无排斥和解决排斥类别中包含 IL-2 信息的 15 个活检中，有 9 个在不久后继续显示排斥。 IL-2 和 IL-5 基因表达很少在同种异体移植物中同时发生。
IL-2 and IL-5 gene expression in response to alloantigen was studied in liver allograft recipients and in an in vitro system. Seventy-seven sequential liver allograft biopsies from 22 patients were analyzed for IL-2 and IL-5 mRNA by polymerase chain reaction and Southern blot hybridization. Message for IL-5 was present in 74% of allografts with rejection, 46% of allografts with resolving rejection, and 33% of allografts with no evidence of rejection. The frequency of IL-5 transcripts in rejecting allografts was significantly different than the frequency of IL-5 transcripts in grafts without evidence of rejection (P= 0.003). Message for IL-2 was detected in 29% of rejecting allografts, 18% of allografts without evidence of rejection, and 43% of allografts with resolving rejection. There was no significant association between IL-2 gene expression and the histopathological status of the allograft. Interestingly, 9 of 15 biopsies that contained IL-2 message in the no rejection and resolving rejection categories went on to display rejection shortly thereafter. IL-2 and IL-5 gene expression rarely occurred simultaneously within allografts.