MALTOSE TRANSPORT IN ESCHERICHIA-COLI K-12 - INVOLVEMENT OF BACTERIOPHAGE-LAMBDA RECEPTOR
MALTOSE TRANSPORT IN ESCHERICHIA-COLI K-12 - INVOLVEMENT OF BACTERIOPHAGE-LAMBDA RECEPTOR
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DOI：
10.1128/jb.124.1.112-118.1975
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发表时间：
1975-01-01
影响因子：
3.2
通讯作者：
HOFNUNG, M
中科院分区：
文献类型：
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作者：
SZMELCMAN, S;HOFNUNG, M
lamB（噬菌体 lambda 受体的结构基因）中受影响的突变体在麦芽糖浓度较低（小于或等于 10 muM）时无法利用麦芽糖。在限制麦芽糖浓度的恒化器中生长期间，在存在野生型菌株的情况下选择测试的lamB突变体。转运研究表明，大多数 lamB 突变体在低麦芽糖浓度下麦芽糖转运能力不足。当将针对纯化噬菌体 lambda 受体的抗体添加到野生型菌株中时，低浓度麦芽糖的转运显着减少。这些结果强烈表明噬菌体 lambda 受体分子参与麦芽糖转运。
Mutants affected in lamB, the structural gene for phage lambda receptor, are unable to utilize maltose when it is present at low concentrations (less than or equal 10 muM). During growth in a chemostat at limiting maltose concentrations, the lamB mutants tested were selected against in the presence of the wild-type strain. Transport studies demonstrate that most lamB mutants have deficient maltose transport capacities at low maltose concentrations. When antibodies against purified phage lambda receptor are added to a wild-type strain, transport of maltose at low concentrations is significantly reduced. These results strongly suggest that the phage lambda receptor molecule is involved in maltose transport.