Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene‐Laden Alginate Microfibers
Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene‐Laden Alginate Microfibers
复制标题
神经细胞在制造后以及长时间封装在导电石墨烯和负载海藻酸盐微纤维内后的行为
DOI：
10.1002/adbi.202101026
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Hashemi, Nicole N.
中科院分区：
文献类型：
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作者：
McNamara, Marilyn C.;Aykar, Saurabh S.;Alimoradi, Nima;Niaraki Asli, Amir Ehsan;Pemathilaka, Rajeendra L.;Wrede, Alex H.;Montazami, Reza;Hashemi, Nicole N.
工程导电性3D细胞支架为创建具有集成实时传感功能的生理相关平台提供了优势。多巴胺能神经细胞使用微流控方法被封装到石墨烯负载的海藻酸盐微纤维中，这对于创建高度可调的微纤维是无与伦比的。加入石墨烯后，海藻酸盐微纤维的导电性提高了148%，与天然脑组织的导电性相似。细胞包封程序的效率为50%，并且在整个6天的观察期内，这些细胞中约有30%保留下来。为了了解微流体包封如何影响细胞遗传学，我们首先用实时逆转录-定量聚合酶链反应分析酪氨酸羟化酶、微管蛋白β 3类3、白细胞介素1 β和肿瘤坏死因子α，其次用酶联免疫吸附法分析，在制造后立即进行分析，在聚合物基质中包封6天后，在石墨烯-聚合物复合材料中包封6天后。初步数据表明，制备工艺和与海藻酸盐基质的结合在制备后立即影响了所研究基因的表达。此外，石墨烯的引入进一步改变了基因表达。神经细胞长期包封在海藻酸盐中和6天暴露在石墨烯中也会导致基因表达的变化。
Engineering conductive 3D cell scaffoldings offer advantages toward the creation of physiologically relevant platforms with integrated real‐time sensing capabilities. Dopaminergic neural cells are encapsulated into graphene‐laden alginate microfibers using a microfluidic approach, which is unmatched for creating highly‐tunable microfibers. Incorporating graphene increases the conductivity of the alginate microfibers by 148%, creating a similar conductivity to native brain tissue. The cell encapsulation procedure has an efficiency of 50%, and of those cells, ≈30% remain for the entire 6‐day observation period. To understand how the microfluidic encapsulation affects cell genetics, tyrosine hydroxylase, tubulin beta 3 class 3, interleukin 1 beta, and tumor necrosis factor alfa are analyzed primarily with real‐time reverse transcription‐quantitative polymerase chain reaction and secondarily with enzyme‐linked immunosorbent assay, immediately after manufacturing, after encapsulation in polymer matrix for 6 days, and after encapsulation in the graphene‐polymer composite for 6 days. Preliminary data shows that the manufacturing process and combination with alginate matrix affect the expression of the studied genes immediately after manufacturing. In addition, the introduction of graphene further changes gene expressions. Long‐term encapsulation of neural cells in alginate and 6‐day exposure to graphene also leads to changes in gene expressions.