Spatial and Spectral Super-resolution Imaging for Characterizing Multichromophoric Systems
Spatial and Spectral Super-resolution Imaging for Characterizing Multichromophoric Systems
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DOI：
10.1021/acs.jpcc.0c07790
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发表时间：
2020-11-19
影响因子：
3.7
通讯作者：
Kaufman, Laura J.
中科院分区：
文献类型：
--
作者：
Kim, Hyung Jun;Kwon, Youngah;Kaufman, Laura J.
我们展示了同时基于空间和光谱减法的超分辨率显微镜的基础上，光漂白，光闪烁，或在多发色团系统的光活化，以详细说明在聚合物膜中分离和固定的小聚集体中的单个发射器的特性。特别是，我们研究了原型多色电子给电子系统聚[2-甲氧基-5-（2-乙基己氧基）-1，4-phenylenevinylene]（MEH-PPV）和电子接受N，N '-dipentyl-3，4，9，10-perylenedicarboximide（pPDI）分子制备为小聚集体通过串联分析的空间和光谱信息的单个发射器内的衍射限制点。在MEH-PPV聚集体中，这种方法揭示了链限制和域限制激子迁移以及具有大量分子间接触的区域的优先光降解的证据。在小聚集体的pPDI，个别亚基具有不同的光谱特性和电子耦合特性的证据被发现。
We demonstrate simultaneous spatial and spectral subtraction-based super-resolution microscopy based on photobleaching, photoblinking, or photoactivation in multichromophoric systems to detail the characteristics of individual emitters in small aggregates isolated and immobilized in polymeric films. In particular, we investigate the prototypical multichromophoric electron-donating system poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene] (MEH-PPV) and electron-accepting N,N'-dipentyl-3,4,9,10-perylenedicarboximide (pPDI) molecules prepared as small aggregates via tandem analysis of spatial and spectral information of individual emitters within a diffraction-limited spot. In MEH-PPV aggregates, this approach reveals evidence of both chain-limited and domain-limited exciton migration as well as preferential photodegradation of regions with a high number of intermolecular contacts. In small aggregates of pPDI, evidence of individual subunits with distinct spectral characteristics and electronic coupling properties is found.