Host-pathogen interactions in experimental Mycoplasma pneumoniae disease studied by the freeze-fracture technique.
Host-pathogen interactions in experimental Mycoplasma pneumoniae disease studied by the freeze-fracture technique.
复制标题
通过冷冻断裂技术研究实验性肺炎支原体疾病中宿主与病原体的相互作用。
DOI：
10.1093/clinids/4.supplement_1.s173
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Collier,AM
中科院分区：
文献类型：
--
作者：
Carson,JL;Collier,AM
冷冻断裂和器官培养技术在肺炎支原体致病研究中的应用为研究宿主-病原体相互作用提供了新的视角。M.观察了该病原菌在实验感染过程中引起的宿主细胞膜的变化。研究了M.肺炎显示存在大量的膜相关颗粒嵌入在双分子小叶的内部。然而，细胞膜破裂的区域有时会出现无颗粒区和/或水泡。在器官培养中，这些无颗粒区域有时与宿主气管上皮紧密对齐。此外，观察到实验感染期间宿主上皮细胞膜完整性的显著变化。经常观察到管腔表面膜的恶化和睫状体膜内颗粒分布的改变。纤毛功能障碍也表明观察异常对齐和丛生的相邻纤毛。这些发现与实验期间宿主大分子合成改变和纤毛停滞观察的生化数据相关。肺炎感染
The application of freeze-fracture and organ culture technology to the study of disease caused byMycoplasma pneumoniaeprovided a new perspective on host-pathogen interactions. Structural variations of theM. pneumoniaemembrane and alterations of the host cell membrane that are induced by this pathogen during experimental infection were observed. Study of freeze-fracture preparations ofM. pneumoniaerevealed the presence of numerous membrane-associated particles embedded in the inner aspect of the bimolecular leaflet. However, areas of the fractured membrane of cells sometimes had particle-free zones and/or blebs. These particle-free areas were sometimes closely aligned to host tracheal epithelium in organ culture. In addition, marked changes in the membrane integrity of the host epithelium during experimental infection were observed. Deterioration of luminal surface membranes and alterations in the distribution of ciliary intramembranous particles were frequently noted. Ciliary dysfunction was also suggested by observations of abnormally aligned and clumped adjacent cilia. These findings correlated well with biochemical data demonstrating altered host macromolecular synthesis and observations of ciliostasis during experimentalM. pneumoniaeinfection.