Apical and basal orthodromic population spikes in hippocampal CA1 in vivo show different origins and patterns of propagation
Apical and basal orthodromic population spikes in hippocampal CA1 in vivo show different origins and patterns of propagation
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DOI：
10.1152/jn.2001.86.5.2435
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发表时间：
2001-11-01
影响因子：
2.5
通讯作者：
Leung, LS
中科院分区：
文献类型：
--
作者：
Kloosterman, F;Peloquin, P;Leung, LS
在体内，关于顺向动作电位是起源于CA 1锥体细胞的顶树突还是基底树突存在争议。本文采用同步记录的场电位和电流源密度（CSD）分析方法，研究了在体条件下大鼠海马CA 1区树突参与群体峰电位的起始和传播。CSD分析表明，逆向人口尖峰，刺激引起的肺泡，入侵连续，轴突起始段（层oriens），细胞体和类似的近端顶端树突的200 μ m。CA 1的基底树突的兴奋，刺激后的CA 3层oriens，诱发一个顺向穗开始附近的细胞体或初始段，然后传播到近端顶端树突类似于200妈妈。与此相反，人口穗，随后激发CA 1的顶端树突开始在近端顶端树突，50-100 μ m远侧的细胞体层，然后向心性传播的细胞体和近端基底树突。晚期顶端树突棘可能出现在中间顶端树突（250-300 μ m的细胞层）和远端传播。起源或传播的每一个人口穗类型的模式是相似的近阈值，以超大刺激强度。总之，在体内顶端树突和基底树突兴奋后的群体尖峰似乎起源于不同的位置。顶端树突兴奋诱发的人口尖峰，在近端顶端树突，而基础树突兴奋诱发的尖峰，开始附近的初始段或细胞体。本研究的一个原始发现是在体内从基底树突到顶端树突或反之亦然的群体尖峰的传播。这种从一个树突树到另一个树突树的反向传播可能在CA 3到CA 1神经元网络之间的突触可塑性中发挥重要作用。
There is controversy concerning whether orthodromic action potentials originate from the apical or basal dendrites of CA1 pyramidal cells in vivo. The participation of the dendrites in the initialization and propagation of population spikes in CA1 of urethananesthetized rats in vivo was studied using simultaneously recorded field potentials and current source density (CSD) analysis. CSD analysis revealed that the antidromic population spike, evoked by stimulation of the alveus, invaded in succession, the axon initial segment (stratum oriens), cell body and similar to 200 mum of the proximal apical dendrites. Excitation of the basal dendrites of CA1, following stimulation of CA3 stratum oriens, evoked an orthodromic spike that started near the cell body or initial segment and then propagated similar to 200 mum into the proximal apical dendrites. In contrast, the population spike that followed excitation of the apical dendrites of CA1 initiated at the proximal apical dendrites, 50-100 mum distal to the cell body layer, and then propagated centripetally to the cell body and the proximal basal dendrites. A late apical dendritic spike may arise in the mid-apical dendrites (250-300 mum from the cell layer) and propagated distally. The origin or the pattern of propagation of each population spike type was similar for near-threshold to supramaximal stimulus intensities. In summary, population spikes following apical dendritic and basal dendritic excitation in vivo appeared to originate from different locations. Apical dendritic excitation evoked a population spike that initiated in the proximal apical dendrites while basal dendritic excitation evoked a spike that started near the initial segment or cell body. An original finding of this study is the propagation of the population spike from basal to apical dendrites in vivo or vice versa. This backpropagation from one dendritic tree to the other may play an important role in the synaptic plasticity among a network of CA3 to CA1 neurons.