Alterations in stromal glycoconjugates in macular corneal dystrophy.
Alterations in stromal glycoconjugates in macular corneal dystrophy.
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黄斑角膜营养不良中基质糖缀合物的改变。
DOI：
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发表时间：
1986
影响因子：
4.4
通讯作者：
J. Baum
中科院分区：
文献类型：
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作者：
N. Panjwani;M. Rodrigues;J. Alroy;D. Albert;J. Baum
用9种生物素化凝集素作为组织化学探针，对正常角膜和黄斑性和颗粒性角膜营养不良患者角膜中糖结合物糖残基进行定位。正常角膜和颗粒状营养不良角膜的凝集素结合模式难以区分，但与黄斑营养不良角膜的凝集素结合模式明显不同。刀豆蛋白A与正常角膜基质呈弱反应，而与黄斑营养不良的角膜基质呈强阳性反应。此外，与正常角膜基质不同的是，黄斑营养不良患者的角膜基质与小麦胚凝集素(WGA)、蓖麻凝集素I(RCA-I)、欧洲扁豆凝集素I、扁豆凝集素I、单叶扁豆凝集素II、大豆和花生凝集素呈阳性反应。这项研究证实了黄斑营养不良患者角膜基质中糖结合物的特殊变化，即存在末端含有α-岩藻糖、β-半乳糖、N-乙酰氨基葡萄糖和N-乙酰半乳糖胺残基的寡糖，以及含有β-半乳糖-N-乙酰半乳糖胺序列的寡糖链。
Nine biotinylated lectins were used as histochemical probes to localize the carbohydrates residues of glycoconjugates in normal corneas and in corneas with macular and granular dystrophy. The lectin binding patterns of normal corneas and of corneas with granular dystrophy were indistinguishable from one another, but were distinctly different from those found in corneas with macular dystrophy. Concanavalin A reacted weakly with normal corneal stromal matrix, but stained stromal matrix of corneas with macular dystrophy intensely. Furthermore, unlike the normal corneal matrix, stromal matrix of corneas with macular dystrophy reacted positively with wheat germ agglutinin (WGA), Ricinus communis agglutinin I (RCA-I), Ulex europeus I, Dolichos biflorus, Bandeiraea simplicifolia I, Bandeiraea simplicifolia II, and soybean and peanut lectins. This study demonstrates specific alterations in glycoconjugates which occur in the corneal matrix of patients with macular dystrophy, namely the presence of oligosaccharides with terminal alpha-fucose, beta-galactose, N-acetylglucosamine and N-acetylgalactosamine residues, and oligosaccharide chains with a beta-galactose-N-acetylgalactosamine sequence.
黄斑角膜营养不良——粘多糖代谢的局部紊乱？
DOI：
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发表时间：
1982
期刊：
Progress in clinical and biological research
影响因子：
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作者：
Klintworth,GK
通讯作者：
Klintworth,GK