Transactivation of human immunodeficiency virus by herpesviruses.
Transactivation of human immunodeficiency virus by herpesviruses.
复制标题
疱疹病毒对人类免疫缺陷病毒的反式激活。
作者：
R. Rando;P. Pellett;P. Luciw;C. Bohan;A. Srinivasan
我们研究了人类免疫缺陷病毒（HIV）和疱疹病毒的相互作用。为此，构建了嵌合质粒（pLTR-CAT），其中来自HIV分子克隆的长末端重复（LTR）序列融合到细菌氯霉素乙酰转移酶基因（CAT）。使用转染的组织培养细胞中的瞬时表达测定来监测LTR的活性。用pLTR-CAT转染HeLa细胞和人肺成纤维细胞（HLF），检测CAT活性的基础水平。用疱疹病毒感染HeLa细胞和HLF细胞，检测到HIV-LTR介导的CAT基因表达。对于单纯疱疹病毒（HSV）-1和HSV-2、巨细胞病毒和水痘带状疱疹病毒，观察到HIV LTR指导的CAT表达增强。通过共转染含有HSV-1的两个非重叠区域、来自HSV-2的与来自HSV-1的所用区域非共线的片段、伪狂犬病病毒的立即早期基因和腺病毒早期基因EIA的重组质粒，也显示了由LTR指导的增强的CAT表达。HIV LTR定向表达可能是研究已知存在于AIDS或HIV感染个体中的各种感染因子对HIV的影响的有用模型。
We examined the interaction of human immunodeficiency virus (HIV) and herpes group viruses. For this purpose, a chimeric plasmid (pLTR-CAT) was constructed in which the long terminal repeat (LTR) sequences derived from a molecular clone of HIV were fused to a bacterial chloramphenicol acetyltransferase gene (CAT). Transient expression assays in transfected tissue culture cells were used to monitor the activity of the LTR. Basal levels of CAT activity were measured in HeLa and human lung fibroblast (HLF) cells transfected with pLTR-CAT. When HeLa or HLF cells transfected with pLTR-CAT were infected with herpesviruses, HIV LTR-directed expression of the CAT gene was detected. An enhancement of the HIV LTR-directed expression of CAT was observed for herpes simplex virus (HSV)-1 and HSV-2, cytomegalovirus and varicella zoster virus. Enhanced CAT expression directed by the LTR was also shown by cotransfection of recombinant plasmids containing two non-overlapping regions of HSV-1, a fragment from HSV-2 which is non-colinear with the regions used from HSV-1, the immediate early gene of pseudorabies virus and the adenovirus early gene EIA. HIV LTR-directed expression may be a useful model for studying the effects on HIV of various infectious agents known to be present in individuals with AIDS or HIV infection.