NUCLEOTIDE-SEQUENCE AND CELLULAR-DISTRIBUTION OF RAT CHROMOGRANIN-B (SECRETOGRANIN-I) MESSENGER-RNA IN THE NEURO-ENDOCRINE SYSTEM
NUCLEOTIDE-SEQUENCE AND CELLULAR-DISTRIBUTION OF RAT CHROMOGRANIN-B (SECRETOGRANIN-I) MESSENGER-RNA IN THE NEURO-ENDOCRINE SYSTEM
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DOI：
10.1007/bf02896890
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发表时间：
1989-01-01
影响因子：
3.1
通讯作者：
SUTCLIFFE, JG
中科院分区：
文献类型：
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作者：
FORSSPETTER, S;DANIELSON, P;SUTCLIFFE, JG
大鼠分泌囊泡蛋白嗜铬粒蛋白B mRNA在脑组织、肾上腺髓质和垂体前叶中含量丰富。从这个2,337个核苷酸的转录物的cDNA序列中预测的初级翻译产物对应于一个由信号肽处理的亲水性655残基蛋白。成熟75-kD蛋白的两端与其他嗜铬粒蛋白具有广泛的相似性；更可变的内部区域的特点是谷氨酸簇和许多对基本残基。在啮齿类动物的大脑中，mRNA的积累始于胚胎13-14天，在出生后第20天达到峰值。脑切片原位杂交显示，mRNA在海马区、下丘脑内分泌区、嗅觉系统和脑桥-延髓的解剖结构中富集。
The mRNA of rat secretory-vesicle protein chromogranin B is abundant in brain, adrenal medulla, and anterior pituitary. The primary translation product predicted from the cDNA sequence of this 2,337-nucleotide transcript corresponds to a hydrophilic 655-residue protein proceded by a signal peptide. Both termini of the mature 75-kD protein show extensive similarity to other chromogranins; the more variable internal region is characterized by glutamic acid clusters and numerous pairs of basic residues. In rodent brain, mRNA accumulation starts around embryonic days 13-14 and peaks by post-natal day 20. In situ hybridization in brain sections shows that the mRNA is enriched in the hippocampal formation, the endocrine hypothalamus, the olfactory system, and in anatomically distinct structures in the pons-medulla.