Rabbit Model for in vivo Study of Intervertebral Disc Degeneration and Regeneration
Rabbit Model for in vivo Study of Intervertebral Disc Degeneration and Regeneration
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DOI：
10.3340/jkns.2008.44.5.327
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发表时间：
2008-11-01
影响因子：
1.6
通讯作者：
Wang, Jeffrey C.
中科院分区：
文献类型：
--
作者：
Kong, Min Ho;Do, Duc H.;Wang, Jeffrey C.
目的 本研究旨在验证兔模型对椎间盘退变研究的实用性。材料采用16号针在左前外侧纤维环中插入5 mm深度，同时保留L5-L6 IVD，对9只成年雄性新西兰白兔的L1-L2、L2-L3、L3-L4或L4-L5腰椎间盘（IVD）进行损伤。没有受伤。另外三只兔子除了在 L1-L2 水平受到 16 号针损伤外，还在 L4-L5 接受了转染腺病毒和骨形态发生蛋白 2 (ad-BMP-2) 的兔椎间盘细胞的椎间内注射。使用数字化射线照片，使用椎间盘高度指数 (DHI) 进行 IVD 高度测量和分析。术后 15 周对受伤椎间盘、注射椎间盘和未受伤的 L5-L6 椎间盘进行磁共振成像 (MRI) 扫描，并与术前 MRI 扫描进行比较。 结果：环形穿刺后 15 周内，所有 12 只兔子都显示出一致的椎间盘退变结果。受伤椎间盘的 DHIS 显着低于未受伤的 L5L6 椎间盘 (p < 0.05)。受伤椎间盘的椎间盘退变等级平均值显着高于未受伤椎间盘（p < 0.05）。注射转染ad-BMP-2的椎间盘细胞在注射后15周没有诱导椎间盘再生。结论：本研究表明，15周内所有兔子的损伤椎间盘在简单侧位片上的DHI和MRI扫描上的椎间盘退变等级都有显着变化。兔环形穿刺模型可用作体内椎间盘退变模型。
Objective The purpose of this study is to verify the usefulness of the rabbit model for disc degeneration study.Materials The L1-L2, L2-L3, L3-L4, or L4-L5 lumbar intervertebral disc (IVD) of 9 mature male New Zealand White rabbits were injured by inserting a 16-gauge needle to a depth of 5 mm in the left anterolateral annulus fibrosus while leaving L5-L6 IVD uninjured. Three other rabbits also received intradiscal injections of rabbit disc cells transfected with adenovirus and bone morphogenetic protein-2 (ad-BMP-2) at L4-L5 in addition to injury by 16-gauge needle at the L1-L2 level. Using digitized radiographs, measurements of IVD height were made and analyzed by using the disc height index (DHI). Magnetic resonance imaging (MRI) scans of the injured discs, injected discs, and uninjured L5-L6 discs were performed at 15 weeks post surgery and compared with preoperative MRI scans.Results : All twelve rabbits showed consistent results of disc degeneration within 15 weeks following annular puncture. DHIS of injured discs were significantly lower than that of the uninjured L5L6 discs (p < 0.05). The mean value of disc degeneration grade of injured discs was significantly higher than that of uninjured discs (p < 0.05). The injection of disc cell transfected with ad-BMP-2 did not induce disc regeneration at 15 weeks after injection.Conclusion : This study showed that the injured disc had a significant change in DHI on simple lateral radiograph and disc degeneration grade on MRI scans within 15 weeks in all rabbits. Rabbit annular puncture model can be useful as a disc degeneration model in vivo.