Combinatorial stresses kill pathogenic Candida species.
Combinatorial stresses kill pathogenic Candida species.
复制标题
DOI：
10.3109/13693786.2012.672770
复制
发表时间：
2012-10
期刊：
影响因子：
2.9
通讯作者：
Brown AJ
中科院分区：
文献类型：
--
作者：
Kaloriti D;Tillmann A;Cook E;Jacobsen M;You T;Lenardon M;Ames L;Barahona M;Chandrasekaran K;Coghill G;Goodman D;Gow NA;Grebogi C;Ho HL;Ingram P;McDonagh A;de Moura AP;Pang W;Puttnam M;Radmaneshfar E;Romano MC;Silk D;Stark J;Stumpf M;Thiel M;Thorne T;Usher J;Yin Z;Haynes K;Brown AJ
病原微生物存在于动态的生态位中，并进化出强大的适应反应来促进宿主的生存。白色念珠菌和光滑念珠菌是人类的主要真菌病原体，它们在宿主体内暴露于一系列环境应激，包括渗透胁迫、氧化应激和亚硝化应激。对每一种压力的适应性反应进行了大量的研究。在野外，细胞经常同时暴露在这些压力的组合中，但这种组合压力的影响尚未被探索。我们开发了一个通用的实验平台，以便于比较光滑念珠菌和白色念珠菌的组合应激反应。该平台基于细胞在30°C的富缓冲介质中的生长，用于定义相对低、中、高剂量的渗透(NaC L)、氧化(H2 O2)和亚硝化应激(例如，二丙三胺(DPTA)-NOate)。在这些生长条件下，将组合应力的影响与相应的单独应力进行了比较。我们首次证明，某些组合的压力在杀死白色念珠菌和光滑念珠菌和/或抑制它们的生长方面特别有效。渗透压加氧化应激和氧化加亚硝化应激的组合就是这种情况。我们预测，如果不能在宿主防御这些病原酵母的情况下，组合应激可能是非常重要的。
Pathogenic microbes exist in dynamic niches and have evolved robust adaptive responses to promote survival in their hosts. The major fungal pathogens of humans, Candida albicans and Candida glabrata, are exposed to a range of environmental stresses in their hosts including osmotic, oxidative and nitrosative stresses. Significant efforts have been devoted to the characterization of the adaptive responses to each of these stresses. In the wild, cells are frequently exposed simultaneously to combinations of these stresses and yet the effects of such combinatorial stresses have not been explored. We have developed a common experimental platform to facilitate the comparison of combinatorial stress responses in C. glabrata and C. albicans. This platform is based on the growth of cells in buffered rich medium at 30°C, and was used to define relatively low, medium and high doses of osmotic (NaCl), oxidative (H 2O2) and nitrosative stresses (e.g., dipropylenetriamine (DPTA)-NONOate). The effects of combinatorial stresses were compared with the corresponding individual stresses under these growth conditions. We show for the first time that certain combinations of combinatorial stress are especially potent in terms of their ability to kill C. albicans and C. glabrata and/or inhibit their growth. This was the case for combinations of osmotic plus oxidative stress and for oxidative plus nitrosative stress. We predict that combinatorial stresses may be highly signif cant in host defences against these pathogenic yeasts.
登录
查看更多内容
影响因子：
5.4
作者：
Brown, Alistair J. P.;Haynes, Ken;Quinn, Janet
通讯作者：
Quinn, Janet
影响因子：
--
作者：
Chiranand, Wiriya;McLeod, Ian;Gustin, Michael C.
通讯作者：
Gustin, Michael C.
DOI：
10.1073/pnas.0611195104
发表时间：
2007-05-01
影响因子：
11.1
作者：
Kaur, Rupinder;Ma, Biao;Cormack, Brendan P.
通讯作者：
Cormack, Brendan P.
影响因子：
2.9
作者：
Gross, NT;Nessa, K;Jarstrand, C
通讯作者：
Jarstrand, C
影响因子：
3.6
作者：
HRABIE, JA;KLOSE, JR;KEEFER, LK
通讯作者：
KEEFER, LK