Transcriptional regulation of salivary proline-rich protein gene expression.
Transcriptional regulation of salivary proline-rich protein gene expression.
复制标题
唾液富含脯氨酸蛋白基因表达的转录调控。
DOI：
10.1111/j.1749-6632.1998.tb09638.x
复制
发表时间：
1998
影响因子：
5.2
通讯作者：
Lin,HH
中科院分区：
文献类型：
--
作者：
Ann,DK;Lin,HH
翻译后摘要：真核生物中的基因表达和调控机制是非常复杂的。从体内转基因和体外转染研究的结果，旨在确定顺式元件（S）和反式因子（S）与唾液富含脯氨酸的蛋白质（PRPs）基因的表达被用作一个范例来讨论唾液特异性基因表达的调控。特别关注的是唾液PRPR 15基因调控的分子机制。在啮齿类动物中，PRP选择性地在唾液腺的腺泡细胞中表达，并且可由β-激动剂异丙肾上腺素以及膳食单宁诱导。从一系列的实验中使用嵌合报告构建体含有不同长度的theR 15远端增强子区域，它们的突变，和各种表达构建体的结果进行了分析和讨论。这些数据表明，诱导型核孤儿受体NGFI-B可能通过三个不同的远端NGFI-B位点参与调节大鼠R15基因的唾液腺泡细胞特异性和诱导型表达。综上所述，提出了异丙肾上腺素诱导R15基因表达的模型。然而，这种NGFI-B-介导的cAMP-调节的R15表达的反式激活的确切分子基础仍有待建立。
Abstract:Mechanisms governing gene expression and regulation in eukaryotes are remarkably complex. The results fromin vivotransgenic andin vitrotransfection studies designed to identifycis‐element(s) andtrans‐factor(s) associated with the salivary proline‐rich proteins (PRPs) gene expression are utilized as a paradigm to discuss the regulation of salivary‐specific gene expression. Particular attention is given to the molecular mechanism(s) underlying the salivary PRPR15gene regulation. In rodents, the PRPs are selectively expressed in the acinar cells of salivary glands, and are inducible by the β‐agonist isoproterenol as well as by dietary tannins. The results from a series of experiments using chimeric reporter constructs containing different lengths of theR15distal enhancer region, their mutations, and various expressing constructs are analyzed and discussed. These data suggest that the inducible nuclear orphan receptor NGFI‐B may participate in the regulation of salivary acinar cell‐specific and inducible expression of the ratR15gene via three distinct distal NGFI‐B sites. Taken together, a model for the induction ofR15gene expression by isoproterenol is proposed. However, the exact molecular basis of this NGFI‐B‐mediated transactivation of cAMP‐regulatedR15expression remains to be established.