I. In vitrol transformation of rat embryo cells: correlations with the known tumorigenic activities of chemicals in rodents.
I. In vitrol transformation of rat embryo cells: correlations with the known tumorigenic activities of chemicals in rodents.
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I. 大鼠胚胎细胞的体外转化：与啮齿动物中已知的化学物质致瘤活性的相关性。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
P. Price
中科院分区：
文献类型：
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作者：
A. Freeman;H. Igel;P. Price
随着大鼠胚胎细胞培养物的传代，对化学诱导转化的敏感性发生变化。对于前35至60代，培养物是二倍体，对化学致癌物的转化具有抗性。然而，用鼠白血病病毒感染的培养物被化学物质转化。对于接下来的60次传代，培养物是异倍体，但保留接触抑制并且不致瘤。即使不添加异型病毒，这些异倍体培养物也可以被化学物质转化，但在转化的培养物中可以证明内源性大鼠C型病毒。在较高的传代次数下，自发转化率逐渐增加，因此培养物不能用于转化研究。化学诱导转化的稳定异倍体细胞系（F1706）表现为一个易于阅读的局灶性改变。通过将形态学改变的细胞移植到同基因新生大鼠中，证实了基于这些病灶的初步观察结果。一些致癌性和非致癌性的化学类似物进行了测试，他们的能力，转化F1706文化。测试的化合物包括4种偶氮染料，12种多环烃，12种芳香胺和7种其他化合物。根据相同化学品在啮齿动物中的已知活性，某些活性化合物在任何试验中均不能诱导转化，而其他化合物仅在某些试验中诱导转化，但这些体外试验如果用作筛选试验，则在所有单独试验中的82%中是正确的，总体而言，将正确预测35种受试制剂中33种的致癌活性。
Susceptibility to chemically induced transformation changed as a rat embryo cell culture was passaged. For the first 35 to 60 passages, the cultures were diploid and resistant to transformation by chemical carcinogens. However, cultures infected with a murine leukemia virus were transformed by chemicals. For the next 60 passages, the cultures were heteroploid, but retained contact inhibition and were not tumorigenic. Even without addition of heterotypic viruses, these heteroploid cultures could be transformed by chemicals, but the endogenous rat C-type virus could be demonstrated in the transformed cultures. At higher passages, the rates of spontaneous transformation gradually increased so that the cultures could not be used for transformation studies. Chemically induced transformation of the stable heteroploid cell line (F1706) was manifested by an easy to read focal alteration. Initial observations based on these foci were confirmed by inoculating the morphologically altered cells into isogeneic newborn rats. A number of carcinogenic and noncarcinogenic chemical analogues were tested for their ability to transform F1706 cultures. The compounds tested included 4 azo dyes, 12 polycyclic hydrocarbons, 12 aromatic amines, and 7 miscellaneous compounds. Based on the known activities of the same chemicals in rodents, certain active compounds failed to induce transformation in any test, and others induced transformation in only some tests, but these in vitro tests, if used as a screening assay, would have been correct in 82% of all individual tests, and over-all, would have correctly predicated the carcinogenic activity of 33 of the 35 agents tested.