Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.
Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.
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含有不同长度的 Poly(A) 片段的球蛋白 mRNA 种类。
DOI：
10.1111/j.1432-1033.1976.tb10279.x
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
Hubert Chantrenne
中科院分区：
文献类型：
--
作者：
Uri Nudel;H. Soreq;Uriel Z. Littauer;Gerard Marbaix;G. Huez;Madeleine Leclercq;Evelyne Hubert;Hubert Chantrenne
采用大肠杆菌多核苷酸磷酸化酶对含有不同长度聚(A)片段的兔珠蛋白mRNA进行部分磷酸化。通过改变盐浓度和磷解混合物的孵育时间，以及在22℃和4℃下进行寡聚(dT)-纤维素色谱，可以获得含有大约122、95、68、39、32、21和16个腺苷酸残基的聚(A)片段的珠蛋白mRNA制剂。结果表明，含有32个及以上腺苷酸残基的mRNA注入非洲爪蟾卵母细胞后，其功能稳定性与天然珠蛋白mRNA相当。另一方面，平均含有21个腺苷酸残基的mRNA的功能稳定性约为天然mRNA的30%，而含有16个腺苷酸残基的mRNA的功能稳定性与无聚(A)珠蛋白mRNA一样低。
Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)-cellulose chromatography at 22 degrees C and at 4 degrees C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as poly(A)-free globin MRNA.