Hydrogels in Cell-Based Therapies
Hydrogels in Cell-Based Therapies
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水凝胶在细胞疗法中的应用
DOI：
10.1039/9781782622055-00135
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Wright B
中科院分区：
文献类型：
--
作者：
Wright B
藻酸盐水凝胶的生物物理性质和生物相容性显著依赖于其来源、提取方法、聚合物组成和凝胶化化学。从各种褐藻（海藻，例如瘤状囊藻（Ascophyllum nodosum）、海带属（Laminaria spp.）Lessonia nigrescens、Ecklonia maxima、Macrocystis pyrifera和Durvillaea anastritica）1、2和某些细菌物种（例如假单胞菌属和固氮菌属）3在b-D-甘露糖醛酸（M）和a-L-古罗糖醛酸（G）含量方面不同。藻酸盐凝胶的机械强度、弹性和溶胀特性根据M：G比率、MMM、GGG和MGM聚合物嵌段的长度以及这些嵌段结构的百分比而变化。用于交联M和G聚合物上的羧基以形成藻酸盐凝胶的多价阳离子（例如Ca 2+、Ba 2+、La 3+、Fe 3+、Zn 2+、Mg 2+、Sr 2+）也决定机械强度。此外，海藻酸多糖的提取影响海藻酸凝胶的粘度，这一性质强烈影响其生物相容性和临床应用。这些物理性质必须共同考虑，以产生适合于生物医学应用的藻酸盐水凝胶。
The biophysical properties and biocompatibility of alginate hydrogels are signi cantly dependent on their source, extraction methodology, polymer composition, and gelation chemistry. Alginates isolated from various brown algae (seaweed, eg Ascophyllum nodosum, Laminaria spp., Lessonia nigrescens, Ecklonia maxima, Macrocystis pyrifera and Durvillaea antarctica) 1, 2 and certain bacterial species (eg Pseudomonas and Azotobacter genus) 3 are dissimilar with regard to b-D-mannuronic acid (M) and a-L-guluronic acid (G) content. Alginate gel mechanical strength, elasticity and swelling characteristics vary depending on the M: G ratio, length of MMM, GGG and MGM polymer blocks, and the percentage of these block structures. Multivalent cations (eg Ca2+, Ba2+, La3+, Fe3+, Zn2+, Mg2+, Sr2+) used to crosslink carboxyl groups on M and G polymers to form alginate gels also determine mechanical strength. Furthermore, the extraction of alginate polysaccharides affects the viscosity of alginate gels, a property that strongly in uences their biocompatibility and clinical application. These physical properties must be considered collectively to produce alginate hydrogels that are suitable for biomedical application.