Anti-inflammatory effect of Lycium Fruit water extract in lipopolysaccharide-stimulated RAW 264.7 macrophage cells
Anti-inflammatory effect of Lycium Fruit water extract in lipopolysaccharide-stimulated RAW 264.7 macrophage cells
复制标题
DOI：
10.1016/j.intimp.2012.03.020
复制
发表时间：
2012-06-01
影响因子：
5.6
通讯作者：
Ma, Jin Yeul
中科院分区：
文献类型：
--
作者：
Oh, You-Chang;Cho, Won-Kyung;Ma, Jin Yeul
枸杞子在东亚国家被用作治疗腰痛和慢性咳嗽的传统药物。然而，枸杞子水提取物（LFWE）对炎症的抑制作用仍然未知。在这项研究中，我们研究了LFWE对脂多糖（LPS）刺激的RAW 264.7细胞中促炎介质产生的抑制作用。LFWE可抑制LPS诱导的一氧化氮（NO）、前列腺素（PG）E-2、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的产生及其合成酶诱导型一氧化氮合酶（iNOS）和环氧合酶（考克斯）-2基因的表达。此外。LFWE抑制细胞外信号调节激酶（ERK）、p38和c-Jun NH 2-末端激酶（JNK）丝裂原活化蛋白激酶（MAPK）的磷酸化，以及抑制I κ B α降解和核因子（NF）-κ B在LPS刺激下的核转位。此外，LFWE抑制LPS刺激的腹腔巨噬细胞中NO、PGE（2）、TNF-α和IL-6的产生。综上所述，我们的研究结果表明，LFWE通过阻断MAPK和NF-κ B途径抑制各种炎症介质的产生。这一发现首次解释了LFWE在LPS刺激的巨噬细胞中抗炎作用的机制。(C)2012爱思唯尔有限公司版权所有。
Lycium Fruit has been used as a traditional drug for low back pain and chronic cough in east-Asian countries. However, inhibitory effects of Lycium Fruit water extract (LFWE) on inflammation remain unknown. In this study, we investigated the inhibitory effects of LFWE on pro-inflammatory mediator production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. LFWE inhibited LPS-induced nitric oxide (NO), prostaglandin (PG) E-2, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 production as well as their synthesizing enzyme inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 gene expression. Furthermore. LFWE inhibited phosphorylations of extracellular signal-regulated kinase (ERK), p38 and c-Jun NH2-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs) as well as suppression of I kappa B alpha degradation and nuclear translocation of nuclear factor (NF)-kappa B upon LPS stimulation. In addition, LFWE suppressed NO, PGE(2), TNF-alpha and IL-6 production in LPS-stimulated peritoneal macrophage cells. Taken together, our results suggest that LFWE inhibits the production of various inflammatory mediators via blockade on the MAPKs and NF-kappa B pathways. This finding first explains the mechanism of anti-inflammatory effect by LFWE in LPS-stimulated macrophage cells. (C) 2012 Elsevier B.V. All rights reserved.