Alignment of biological macromolecules in novel nonionic liquid crystalline media for NMR experiments
Alignment of biological macromolecules in novel nonionic liquid crystalline media for NMR experiments
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DOI：
10.1021/ja001068h
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发表时间：
2000-08-16
影响因子：
15
通讯作者：
Otting, G
中科院分区：
文献类型：
--
作者：
Rückert, M;Otting, G
正烷基-聚乙二醇/正烷基醇和葡萄糖酮/正己醇混合物在水溶液中形成稀液晶相，适合于生物大分子在磁场中的部分排列。所述成分化合物是市售的且价格低廉。聚乙二醇基体系不带电，因此对pH值不敏感，对盐不敏感，对高蛋白质浓度耐受。它们被证明适用于测量蛋白质、DNA和蛋白质/DNA复合体中的残余偶极偶联。探讨了不同混合物的层状相相对于温度的稳定性范围，并为这些溶致体系在核磁共振光谱中的使用提供了指导。
n-Alkyl-poly(ethylene glycol)/n-alkyl alcohol and glucopone/n-hexanol mixtures are shown to form dilute liquid crystalline phases in aqueous solution which are suitable for partial alignment of biological macromolecules in a magnetic field. The constituent compounds are commercially available and inexpensive. The poly(ethylene glycol)-based systems are uncharged and thus insensitive with respect to pH, little sensitive with respect to salt, and tolerant against high protein concentrations. They are demonstrated to be suitable for measurements of residual dipolar couplings in proteins, DNA, and a protein/DNA complex. The stability range of the lamellar phase with respect to temperature was explored for different mixtures and guidelines are provided for the use of these lyotropic systems in NMR spectroscopy.