FOXO genes in channel catfish and their response after bacterial infection
FOXO genes in channel catfish and their response after bacterial infection
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斑点叉尾鮰的FOXO基因及其细菌感染后的反应
DOI：
10.1016/j.dci.2019.03.010
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发表时间：
2019-08-01
影响因子：
2.9
通讯作者：
Liu, Zhanjiang
中科院分区：
文献类型：
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作者：
Gao, Lei;Yuan, Zihao;Liu, Zhanjiang
FOXO蛋白是在寿命调节中起关键作用的转录因子叉头家族的一个亚组。此外，FOXO蛋白也参与免疫反应。对斑点叉尾（Ictalurus punctatus）的FOXO基因进行了系统的研究，鉴定出FOXO 1a、FOXO 1b、FOXO 3a、FOXO 3b、FOXO 4、FOXO 6a和FOXO 6b等7个基因。通过系统发育和共线分析，发现FOXO 1，FOXO 3和FOXO 6在鲶鱼基因组中重复，在斑马鱼基因组中。非同义（dN）和同义（dS）取代的相对比率的分析表明，FOXO基因在全球范围内强烈限制负选择。在爱德华氏菌和柱状黄杆菌感染后，在大多数FOXO基因中观察到差异表达模式。在E.在蜱虫感染后，哺乳动物物种中具有直系同源物的四种FOXO基因显著上调，其中FOXO 6b上调最显著。然而，F.感染后，几乎所有FOXO基因的表达水平均未受到显著影响。这些结果表明，无论是病原特异性模式或组织特异性模式存在于鲶鱼后，这两种细菌感染。综上所述，这些发现表明FOXO基因可能在鲶鱼对细菌感染的免疫反应中发挥重要作用。
FOXO proteins are a subgroup of the forkhead family of transcription factors that play crucial roles in lifespan regulation. In addition, FOXO proteins are also involved in immune responses. After a systematic study of FOXO genes in channel catfish, Ictalurus punctatus, seven FOXO genes were identified and characterized, including FOXO1a, FOXO1b, FOXO3a, FOXO3b, FOXO4, FOXO6a and FOXO6b. Through phylogenetic and syntenic analyses, it was found that FOXO1, FOXO3 and FOXO6 were duplicated in the catfish genome, as in the zebrafish genome. Analysis of the relative rates of nonsynonymous (dN) and synonymous (dS) substitutions revealed that the FOXO genes were globally strongly constrained by negative selection. Differential expression patterns were observed in the majority of FOXO genes after Edwardsiella ictaluri and Flavobacterium columnare infections. After E. ictaluri infection, four FOXO genes with orthologs in mammal species were significantly upregulated, where FOXO6b was the most dramatically upregulated. However, after F. columnare infection, the expression levels of almost all FOXO genes were not significantly affected. These results suggested that either a pathogenesis-specific pattern or tissue-specific pattern existed in catfish after these two bacterial infections. Taken together, these findings indicated that FOXO genes may play important roles in immune responses to bacterial infections in catfish.