Trace elements content in semen and their interactions with sperm quality and RedOx status in freshwater fish Cyprinus carpio: A correlation study
Trace elements content in semen and their interactions with sperm quality and RedOx status in freshwater fish Cyprinus carpio: A correlation study
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DOI：
10.1016/j.jtemb.2018.08.005
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发表时间：
2018-01-01
影响因子：
3.5
通讯作者：
Massanyi, Peter
中科院分区：
文献类型：
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作者：
Kovacik, Anton;Tirpak, Filip;Massanyi, Peter
本研究的目的是探讨微量元素含量和红牛状态之间的相互作用，以及精子质量参数（活力特征，DNA片段化）在鱼类精子在自然条件下。本研究使用鲤鱼品种繁殖成熟的雄性淡水鱼（n = 16）。采用电感耦合等离子体发射光谱法（ICP-OES）和冷原子吸收光谱法（CV-AAS）测定鱼糜中微量元素的含量。通过计算机辅助精子分析（CASA）定量测定精子浓度、总活力、前向活力、距离平均路径、距离曲线、距离直线、速度平均路径、速度曲线、速度直线、直线度、直线度、头部横向位移幅度和搏动交叉频率等参数，实现精子质量评价。精液中微量金属元素含量的总体下降趋势为：Zn > Fe > Cu > As > Sr > Ni > Mn > Se > Pb > Cr > Cd > Hg。精子的总活动率较高（91.45%），但前向活动率不到此值的一半（39.47%）。精子DNA片段化值相对较低（4.00-6.29%）。不动精子的百分比显示出与所有RedOx状态参数以及DNA片段化的显著相关性。微量元素（Mn，Se，Sr和Zn）和一些定性精子参数（速度和距离参数）之间存在统计学显著正相关。Cu和Hg含量与前向运动表现出类似的负相关性。汞还与丙二醛的产生相互作用。总体而言，本研究建议在评估潜在的生殖风险时应用环境相关微量元素浓度的多组分混合物。
Objective of the present study was to investigate interactions between trace elements content and RedOx status, as well as sperm quality parameters (motility features, DNA fragmentation) in fish spermatozoa in natural conditions. Reproductively mature male freshwater fish (n = 16) of Cyprinus carpio breed were used in the study. Trace elements content was determined in fish milt samples by inductively-coupled plasma optical emission spectrometry (ICP-OES) and by cold-vapor atomic absorption spectroscopy (CV-AAS). Sperm quality evaluation was realized by computer-assisted sperm analysis (CASA) quantifying several parameters: concentration, total motility, progressive motility, distance average path, distance curved line, distance straight line, velocity average path, velocity curved line, velocity straight line, straightness, linearity, amplitude of lateral head displacement and beat cross frequency. The general scheme of descending concentrations of trace metals in semen samples was following: Zn > Fe > Cu > As > Sr > Ni > Mn > Se > Pb > Cr > Cd > Hg. Total motility of spermatozoa was relatively high (91.45%), however progressive motility was not even half of this value (39.47%). Sperm DNA fragmentation values were relatively low (4.00-6.29%). The percentage of immotile spermatozoa showed a significant correlation with all RedOx status parameters and also with DNA fragmentation. Positive statistically significant correlations were observed between trace elements (Mn, Se, Sr, and Zn) and some qualitative spermatozoa parameters (velocity and distance parameters). Cu and Hg content shows similar negative associations with progressive motility. Hg also interacted with production of malondialdehyde. Overall, the present study suggests application of multi-component mixtures of environmentally related trace elements concentrations when assessing the potential reproductive risk.