A Model of Exposure to Rotavirus from Nondietary Ingestion Iterated by Simulated Intermittent Contacts
A Model of Exposure to Rotavirus from Nondietary Ingestion Iterated by Simulated Intermittent Contacts
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DOI：
10.1111/j.1539-6924.2008.01193.x
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发表时间：
2009-05-01
期刊：
影响因子：
3.8
通讯作者：
Boehm, Alexandria B.
中科院分区：
文献类型：
--
作者：
Julian, Timothy R.;Canales, Robert A.;Boehm, Alexandria B.
现有的微生物风险评估模型很少包含人类与污染物相互作用的详细描述。我们开发了一个随机机制模型暴露于轮状病毒从非饮食摄入迭代模拟间歇口，手口，手口接触典型的6岁以下儿童。随后使用简单的静态剂量-反应关系将这种接触转化为风险。通过实验室实验，我们定量了MS 2噬菌体在玻璃上的平均灭活率（0.0052/hr）和指尖和玻璃之间的平均转移率（36%）。使用这些参数进行的模拟表明，儿童在一小时内从轮状病毒污染的球中摄入的剂量范围为2至1，000个病毒，中位数为42个病毒。这些结果受到选定的模型参数值的影响很大，最明显的是轮状病毒的浓度fomes，频率的fomes-mouth接触，手-口接触的频率，和病毒从fomes转移到口。该模型表明，口含甲醛是主要的暴露途径，在最初的10分钟互动中，手口含的贡献占儿童剂量的不到五分之一。
Existing microbial risk assessment models rarely incorporate detailed descriptions of human interaction with fomites. We develop a stochastic-mechanistic model of exposure to rotavirus from nondietary ingestion iterated by simulated intermittent fomes-mouth, hand-mouth, and hand-fomes contacts typical of a child under six years of age. This exposure is subsequently translated to risk using a simple static dose-response relationship. Through laboratory experiments, we quantified the mean rate of inactivation for MS2 phage on glass (0.0052/hr) and mean transfer between fingertips and glass (36%). Simulations using these parameters demonstrated that a child's ingested dose from a rotavirus-contaminated ball ranges from 2 to 1,000 virus over a period of one hour, with a median value of 42 virus. These results were heavily influenced by selected values of model parameters, most notably the concentration of rotavirus on fomes, frequency of fomes-mouth contacts, frequency of hand-mouth contacts, and virus transferred from fomes to mouth. The model demonstrated that mouthing of fomes is the primary exposure route, with hand mouthing contributions accounting for less than one-fifth of the child's dose over the first 10 minutes of interaction.