Acacetin inhibits the proliferation of Hep G2 by blocking cell cycle progression and inducing apoptosis
Acacetin inhibits the proliferation of Hep G2 by blocking cell cycle progression and inducing apoptosis
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DOI：
10.1016/j.bcp.2003.09.042
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发表时间：
2004-03-01
影响因子：
5.8
通讯作者：
Lin, CC
中科院分区：
文献类型：
--
作者：
Hsu, YL;Kuo, PL;Lin, CC
类黄酮是一类分布广泛的植物色素，普遍存在于维管植物中，并负责自然界的大部分着色。它们是天然存在于食物中的强抗氧化剂，可以抑制啮齿动物的致癌作用。在这项研究中，我们检查了金合欢素（5,7-二羟基-4'-甲氧基黄酮）（一种类黄酮化合物）对人肝癌细胞系 Hep G2 增殖的影响。结果表明，金合欢素通过诱导细胞凋亡和阻断细胞周期进程至G1期来抑制Hep G2的增殖。酶联免疫吸附测定表明，金合欢素显着增加 p53 和 p21/WAF1 蛋白的表达，有助于细胞周期停滞。 Fas/APO-1 及其两种形式配体（膜结合 Fas 配体和可溶性 Fas 配体）的增强。以及 Bax 蛋白，负责金合欢素诱导的细胞凋亡作用。综上所述，我们的研究表明 p53 的诱导和 Fas/Fas 配体凋亡系统的活性可能参与金合欢素在 Hep G2 细胞中的抗增殖活性。 (C) 2003 Elsevier Inc. 保留所有权利。
Flavonoids are a broadly distributed class of plant pigments, universally present in vascular plants and responsible for much of the coloring in nature. They are strong antioxidants that occur naturally in foods and can inhibit carcinogenesis in rodents. In this study, we examined acacetin (5,7-dihydroxy-4'-methoxyflavone), a flavonoid compound, for its effect on proliferation in a human liver cancer cell line, Hep G2. The results showed that acacetin inhibited the proliferation of Hep G2 by inducing apoptosis and blocking cell cycle progression in the G1 phase. Enzyme-linked immunosorbent assay showed that acacetin significantly increased the expression of p53 and p21/WAF1 protein, contributing to cell cycle arrest. An enhancement in Fas/APO-1 and its two form ligands, membrane-bound Fas ligand and soluble Fas ligand. as well as Bax protein, was responsible for the apoptotic effect induced by acacetin. Taken together, our study suggests that the induction of p53 and activity of the Fas/Fas ligand apoptotic system may participate in the antiproliferative activity of acacetin in Hep G2 cells. (C) 2003 Elsevier Inc. All fights reserved.