[18F]tetrafluoroborate-PET/CT enables sensitive tumor and metastasis in vivo imaging in a sodium iodide symporter-expressing tumor model
[18F]tetrafluoroborate-PET/CT enables sensitive tumor and metastasis in vivo imaging in a sodium iodide symporter-expressing tumor model
复制标题
DOI：
10.1038/s41598-017-01044-4
复制
发表时间：
2017-04-19
影响因子：
4.6
通讯作者：
Fruhwirth, G. O.
中科院分区：
文献类型：
--
作者：
Diocou, S.;Volpe, A.;Fruhwirth, G. O.
癌细胞转移是大多数癌症死亡的原因。在自发转移的肿瘤模型中进行非侵入性体内癌细胞追踪仍然是一项挑战，需要最高的灵敏度和出色的对比度。本研究的目的是评估最近引入的PET放射性示踪剂[ F-18]四氟硼酸盐（[ F-18] BF 4-）是否可用于表达人钠碘同向转运体（NIS）作为报告基因的原位异种移植乳腺癌模型中的灵敏度和特异性转移检测。体内成像是由离体荧光显微镜和补充。收获组织的计数。将[F-18] BF 4-（PET/CT）放射性核素成像与常规示踪剂[I-123]碘化物（序贯SPECT/CT）进行比较。我们发现[F-18] BF 4-由于更好的药代动力学而更上级，即肿瘤吸收更快，从循环中清除更快和更完全。[F-18] BF 4-PET也具有高度特异性，因为在所有检测到的组织中，癌细胞的存在都在显微镜下得到证实。同样发现未检测到的可比组织无转移。由于该模型中相邻代谢活性器官引起的低标准摄取值和低对比度，使用[F-18] FDG-PET进行常规代谢成像的转移检测失败。[F-18] BF 4--PET与NIS表达疾病模型的组合在临床前体内细胞追踪感兴趣时特别有用。
Cancer cell metastasis is responsible for most cancer deaths. Non-invasive in vivo cancer cell tracking in spontaneously metastasizing tumor models still poses a challenge requiring highest sensitivity and excellent contrast. The goal of this study was to evaluate if the recently introduced PET radiotracer [ F-18] tetrafluoroborate ([ F-18] BF4-) is useful for sensitive and specific metastasis detection in an orthotopic xenograft breast cancer model expressing the human sodium iodide symporter (NIS) as a reporter. In vivo imaging was complemented by ex vivo fluorescence microscopy and.-counting of harvested tissues. Radionuclide imaging with [F-18] BF4- (PET/CT) was compared to the conventional tracer [I-123] iodide (sequential SPECT/CT). We found that [F-18] BF4- was superior due to better pharmacokinetics, i.e. faster tumor uptake and faster and more complete clearance from circulation. [F-18] BF4--PET was also highly specific as in all detected tissues cancer cell presence was confirmed microscopically. Undetected comparable tissues were similarly found to be free of metastasis. Metastasis detection by routine metabolic imaging with [F-18] FDG-PET failed due to low standard uptake values and low contrast caused by adjacent metabolically active organs in this model. [F-18] BF4--PET combined with NIS expressing disease models is particularly useful whenever preclinical in vivo cell tracking is of interest.