Cytochrome P-450 gene expression in the functional units of the fetal liver.
Cytochrome P-450 gene expression in the functional units of the fetal liver.
复制标题
胎儿肝脏功能单位中细胞色素 P-450 基因的表达。
DOI：
10.1002/hep.1840080222
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Gumucio,JJ
中科院分区：
文献类型：
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作者：
Chianale,J;Dvorak,C;Farmer,DL;Michaels,L;Gumucio,JJ
胎肝左右叶的肝细胞被不同的微环境所包围。胎儿肝脏的右叶和左叶由携带不同浓度氧气的血管系统灌注，并构成不同的功能单位。本研究的目的是评估苯巴比妥诱导的细胞色素P-450 B，e基因在小鼠左右胎肝叶肝细胞中的表达。使用[32 P] cDNA进行北方印迹分析和定量斑点印迹杂交，以评估左右胎肝叶中这些mRNA的大小和水平。在胎鼠中，细胞色素P-450、B、e mRNA水平在左胎肝叶中高于右胎肝叶。在妊娠的最后几天和出生后不久，肝脏细胞色素P-450 B，e mRNA水平主要在左肝叶增加。相比之下，白蛋白和甲胎蛋白mRNA（研究基因以评估这些发现的特异性）的水平在胎肝的每个功能单位中相似。在任一胎仔肝叶中均未观察到苯巴比妥诱导细胞色素P-450 B、e mRNA。出生后，苯巴比妥诱导这些细胞色素类似的右，左肝叶。因此，胎肝细胞周围的微环境似乎影响细胞色素P-450 B，e基因的表达。这种表达的叶异质性随着成人肝循环模式的获得而消失。
Hepatocytes of the right and left lobes of the fetal liver are surrounded by different microenvironments. The right and left lobes of the fetal liver are perfused by vascular systems carrying different concentrations of oxygen and constitute distinct functional units. The aim of this study was to assess the expression of the phenobarbital‐inducible cytochrome P‐450 b,e genes in hepatocytes of the right and left fetal liver lobes in mice. Northern‐blot analysis using [32P]cDNAs and quantitative dot‐blot hybridization were performed to assess the size and levels of these mRNAs in the right and left fetal liver lobes. In fetal mice, the levels of cytochrome P‐450, b,e mRNAs were higher in the left than in the right fetal liver lobe. During the last days of gestation and in the immediate postnatal period, the levels of liver cytochrome P‐450 b,e mRNAs increased predominantly in the left liver lobe. In contrast, the levels of albumin and α‐fetoprotein mRNAs (genes studied to assess the specificity of these findings) were similar in each of functional units of the fetal liver. Phenobarbital induction of cytochromes P‐450 b,e mRNAs was not observed in either of the fetal liver lobes. Postnatally, phenobarbital induced these cytochromes similarly in the right and left liver lobes. Therefore, the microenvironment surrounding fetal hepatocytes seems to influence the expression of the cytochrome P‐450 b,e genes. This lobar heterogeneity of expression disappears as the pattern of adult liver circulation is attained.