Dual-Cues Laden Scaffold Facilitates Neurovascular Regeneration and Motor Functional Recovery After Complete Spinal Cord Injury
Dual-Cues Laden Scaffold Facilitates Neurovascular Regeneration and Motor Functional Recovery After Complete Spinal Cord Injury
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DOI：
10.1002/adhm.202100089
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发表时间：
2021-03-19
影响因子：
10
通讯作者：
Li, Xing
中科院分区：
文献类型：
--
作者：
Liu, Dingyang;Shen, He;Li, Xing
完全横断性脊髓损伤（SCI）严重破坏神经回路和微血管系统的完整性。因此，制造一个功能性的生物支架，可以协调轴突再生和血管重建的病变区域可能会出现一个新的模式，为完整的SCI修复。在这项研究中，光敏水凝胶支架加载胶原结合基质细胞衍生因子-1a和紫杉醇脂质体能够诱导内皮细胞迁移，并促进神经元的轴突生长在体外。此外，当植入大鼠T8完全横断SCI模型时，上述负载双线索的支架在损伤后10天内表现出促进损伤区域轴突和血管再生的协同作用。此外，双线索负载支架植入后也观察到长期治疗效果，包括血管重建，下行和脊髓本体轴突再生，纤维化瘢痕减少，电生理恢复和运动功能改善。综上所述，负载双线索的支架对于重度SCI患者具有良好的临床应用潜力。
Complete transection spinal cord injury (SCI) severely disrupts the integrity of both neural circuits and the microvasculature system. Hence, fabricating a functional bio-scaffold that could coordinate axonal regeneration and vascular reconstruction in the lesion area may emerge as a new paradigm for complete SCI repair. In this study, a photosensitive hydrogel scaffold loaded with collagen-binding stromal cell-derived factor-1a and Taxol liposomes is capable of inducing migration of endothelial cells and promoting neurite outgrowth of neurons in vitro. In addition, when implanted into a rat T8 complete transection SCI model, the above dual-cues laden scaffold exhibits a synergistic effect on facilitating axon and vessel regeneration in the lesion area within 10 days after injury. Moreover, long-term therapeutic effects are also observed after dual-cues laden scaffold implantation, including revascularization, descending and propriospinal axonal regeneration, fibrotic scar reduction, electrophysiological recovery, and motor function improvement. In summary, the dual-cues laden scaffold has good clinical application potential for patients with severe SCI.